天津医药

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食管鳞癌组织及癌细胞株EC9706中COX-2与15-PGDH的表达及临床意义

赵建秋1,卢向东1,2,张志广3,夏秀丽4,钟江鸣4   

  1. 1. 天津医科大学第二医院
    2.
    3. 天津医科大学第二医院消化科
    4. 医大二院
  • 收稿日期:2012-08-06 修回日期:2012-12-11 出版日期:2013-06-15 发布日期:2013-06-15
  • 通讯作者: 赵建秋

The Expression and Clinical Significance of COX-2 and 15-PGDH in Esophageal Cancer and Cancer Cell Strain（EC9706）

ZHAO Jianqiu 1,LU Xiangdong 1,2,ZHANG Zhi guang3,XIA Xiuli 1,ZHONG Jiangming 1   

  1. 1. Department of Gastroenterology, The Second Hospital of Tianjin Medical University
    2.
    3. Department of Gastroenterology,The Second Hospital of Tianjin Medical University
  • Received:2012-08-06 Revised:2012-12-11 Published:2013-06-15 Online:2013-06-15
  • Contact: ZHAO Jianqiu

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摘要： 【摘要】目的 研究食管鳞癌组织及癌细胞株中环氧合酶（COX）-2及15-羟基前列腺素脱氢酶（15-PGDH）的表达及临床意义。方法  采用免疫组化（PV-9000）法检测正常食管黏膜（对照组，n=35)、食管鳞癌组织(n=35)及食管癌细胞株EC9706中COX-2和15-PGDH蛋白表达，RT-PCR法检测食管鳞癌组织及对照组15-PGDH mRNA表达。结果  与对照组比较，食管鳞癌组织COX-2蛋白表达较高，15-PGDH蛋白及15-PGDH mRNA表达减低（均P <0.05）。COX-2、15-PGDH在不同浸润深度、有无淋巴结转移食管鳞癌中比较差异有统计学意义（P < 0.05），在不同性别、年龄及肿瘤分化程度组织中比较差异无统计学意义。食管癌细胞株中COX-2阳性表达，15-PGDH阴性表达。结论  食管鳞癌组织及癌细胞株中COX-2表达增高，15-PGDH表达减少，两者可能参与食管癌的发生、发展。

关键词: 食管肿瘤, 癌, 细胞系, 肿瘤, 环氧合酶-2, 羟前列腺素脱氢酶类, 免疫组织化学, 逆转录聚合酶链反应

Abstract: Objectives:  To study the expression, clinical significance of COX-2 and 15-PGDH in esophageal squamous cell carcinoma and cancer cell strains. Methods : The expressions of COX-2 and 15-PGDH were determined with immunohistochemical (PV-9000) method in normal esophageal mucosa (n=35 patients), esophageal squamous cell carcinoma (n=35 patients), and esophageal cell strains (EC9706). The expression of mRNA of 15-PGDH was determined with RT-PCR method in normal esophageal mucosa and esophageal squamous cell carcinoma. Results:   The expression of COX-2 was significantly higher in esophageal squamous cell carcinoma than in normal tissue (P < 0.01). The expression of 15-PGDH and 15-PGDHmRNA were significantly lower in esophageal squamous cell carcinoma than in normal tissue (P < 0.05，P < 0.01).The expressions of COX-2 and 15-PGDH were significantly associated with invasive depth of carcinoma tissue and the status of lymph node metastasis (P<0.05). The expressions of COX-2 and 15-PGDH were not significantly associated with sex, age and tumor differentiation. A high expression of COX-2 and a low expression of 15-PGDH were observed in esophageal cell strains. Conclusions:  Compared to normal tissue, a higher expression of COX-2 and a lower expression of 15-PGDH were found in esophageal squamous cell carcinoma and cancer cell strains. COX-2 and 15-PGDH may play an important role in the occurrence and development of esophageal carcinoma.

Key words: Esophageal neoplasms, carcinoma, cell line, tumor, Cyclooxygenase-2, hydroxyprostaglandin dehydrogenases, immunohistochemistry, 逆转录聚合酶链反应