天津医药

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Rac1和NF-κB p65的表达与非小细胞肺癌转移及预后的关系

姚培宇1,张逊2,徐美林3,王菁4,韩洪利2,徐医军2   

  1. 1. 天津医科大学研究生院
    2. 天津市胸科医院外科
    3. 天津市胸科医院病理科
    4. 天津胸科医院病理科
  • 收稿日期:2012-10-10 修回日期:2013-01-07 出版日期:2013-06-15 发布日期:2013-06-15
  • 通讯作者: 姚培宇

Correlations between Prognosis, Metastasis and Expression of Rac1 and NF-κB p65 in Non-Small Cell Lung Cancer

YAO Peiyu 1,ZHANGXUN 2,XUMEILIN 3,WANGJING 3,HANHONGLI 2,XUJUNYI 2   

  1. 1. Tianjin Medical University
    2. Department of Thoracic Surgery,Tianjin Chest Hospital
    3. Department of Pathology,Tianjin Chest Hospital
  • Received:2012-10-10 Revised:2013-01-07 Published:2013-06-15 Online:2013-06-15
  • Contact: YAO Peiyu

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摘要： 【摘要】目的  探讨非小细胞肺癌(NSCLC)中Ras相关的C3肉毒素底物1(Rac1)和核转录因子(NF)-κB p65的表达及其临床意义。方法  采用免疫组化Super Pic TureTM Polymer二步法，检测112例石蜡包埋的NSCLC组织及112例相同患者石蜡包埋的正常余肺组织Rac1和NF-κB p65的表达。结果  NSCLC组织中Rac1及NF-κB p65的表达明显高于正常余肺组织，且两者的表达强度在NSCLC组织中呈正相关(r=0.549,P< 0.001)。在肿瘤不同分化程度、TNM分期及淋巴结转移情况的NSCLC患者的Rac1的表达强度差异有统计学意义；在不同TNM分期及淋巴结转移情况的NSCLC患者的NF-κB p65的表达强度差异有统计学意义；而在低分化、高分期及有淋巴结转移的NSCLC患者组织中，2种指标协同表达的现象更容易出现，且差异有统计学意义（P < 0.05）。Rac1高表达组的3年生存率(35.29%)低于低表达组(79.07%)；NF-κB p65高表达组的3年生存率(40.28%)低于低表达组(74.36%)；Rac1与NF-κB p65共同高表达组的3年生存率(37.70%)低于仅1种蛋白高表达(61.11%)及共同低表达者(81.25%)。Rac1和NF-κB p65共同高表达及淋巴结转移是预后的不良因素(P < 0.05)。结论  在NSCLC组织中，Rac1和NF-κB p65的表达呈现较好的相关性，检测两者的表达会对NSCLC患者的临床病理学特征及预后起一定的提示作用。

关键词: NF-κB, 癌, 非小细胞肺, 免疫组织化学, 预后, Kaplan-Meiers评估, Ras相关的C3肉毒素底物1

Abstract: Objective   To investigate expression of Rac1 and NF-κB p65 in Non-Small Cell Lung Cancer(NSCLC) and their clinical significance.Methods   Immunohistochemical staning was applied to detect expression of Rac1 and NF-κB p65 in NSCLC group and control group.Correlations of expression of Rac1 and NF-?B p65 to clinical pathological parameters and prognosis and their correlations were analysed.Result    The expression intensities of Rac1 and NF-κB p65 in NSCLC were distinctly higher than those in control group.A correlation between the expression of Rac1 and NF-κB p65(r=0.656,P<0.001) was also associated with malignancy of NSCLC such as poor differentiation,high TNM stage and lymph node metastasis(P<0.05 or P<0.01). The 3-year survival rates were lower in patients with Rac1 high expression(35.29%) than those with low expression(79.07%) while the 3-year survival rates were lower in patients with NF-κB p65 high expression(40.28%) than those with low expression (74.36%).Further multivariate analysis suggested that both high expression of Rac1 and NF-κB p65 and lymph node metastasis were independent prognostic indicators for NSCLC(P<0.05,P<0.001).Conclusion     The expression of Rac1 in correlates with the expression of NF-κB p65 in NSCLC, which contributes the malignancy-related of NSCLC. Detecting the expression of Rac1 combined with NF-κB p65 in NSCLC tissue may give a clue on prongnosis of patient.

Key words: NF-kappa B, Carcinoma, Non-Small-Cell Lung, immunohistochemistry, prognosis, Kaplan-Meiers estimate, ras-related C3 botulinum toxin substrate 1