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黄芪多糖诱导的树突状细胞疫苗对S180荷瘤小鼠Th1/Th2类细胞因子的影响

荆雪宁1,邱波2,武继彪1,战文翔1   

  1. 1. 山东中医药高等专科学校
    2. 烟台市莱阳中心医院
  • 收稿日期:2014-05-07 修回日期:2014-07-08 出版日期:2014-11-15 发布日期:2014-11-15
  • 通讯作者: 荆雪宁

The inhibitory effect of Dendritic Cell Tumor Vaccine Induced by Astragalus Polysaccharides on the expression of cytokines Th1/Th2 in S180 Tumor-bearing Mice

JING Xue ning1,QIU Bo 2,WU Ji biao1,ZHAN Wen xiang1   

  1. 1. Shandong College of Traditional Chinese Medicine
    2. Yantai Central Hospital of Laiyang City
  • Received:2014-05-07 Revised:2014-07-08 Published:2014-11-15 Online:2014-11-15
  • Contact: JING Xue ning

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摘要：

【摘要】   目的   探讨黄芪多糖诱导成熟的树突状细胞（DC）肿瘤疫苗对 S180 荷瘤小鼠的抗肿瘤作用及作用机制。   方法   体外培养小鼠骨髓来源的 DC， 加入黄芪多糖诱导成熟， 以 S180 肿瘤抗原致敏获得 DC 肿瘤疫苗。 建立S180 荷瘤小鼠模型， 分为模型组、环磷酰胺组、黄芪多糖组、细胞因子组， 在荷瘤第 5 天和第 10 天分别给予相应治疗。 荷瘤第 12 天摘取肿瘤组织、胸腺、脾脏称质量， 计算抑瘤率、胸腺指数、脾脏指数；ELISA 法检测小鼠血清白细胞介素（IL）-4、干扰素（IFN）-γ水平。   结果   黄芪多糖组、细胞因子组的抑瘤率高于环磷酰胺组（64.25%、64.10% vs
35.11%）， 胸腺指数高于环磷酰胺组（1.69±0.26、1.74±0.38 vs 1.45±0.22）， 脾脏指数高于环磷酰胺组(5.44±0.76、5.31±0.81 vs 3.54±0.52)， IL-4 水平(ng/L)低于环磷酰胺组（15.66±2.57、14.72±4.84 vs 23.95±6.07）， IFN-γ水平(ng/L)高于环磷酰胺组（16.54±3.71、17.20±2.03 vs 10.37±2.19）， 差异均有统计学意义。   结论   黄芪多糖诱导的 DC 疫苗可有效发挥抑瘤作用， 其机制可能与提高荷瘤小鼠胸腺指数与脾脏指数， 调节细胞因子表达， 促进 Th1/Th2 失衡向 Th1 细胞占优势的细胞免疫偏移， 增强机体的抗肿瘤免疫功能有关。

关键词: 黄芪多糖, 树突细胞, 白细胞介素4, 干扰素Ⅱ型, 抑瘤率, 胸腺指数, 脾脏指数, Th1/Th2

Abstract:

[Abstract]   Objective   To study the antitumor effects of dendritic cell vaccine induced by astragalus polysaccha?
rides on S180 tumor-bearing mice, and its possible mechanism.   Methods   Dendritic cells derived from mouse bone marrow were induced maturation by astragalus polysaccharides and loaded with S180 tumor antigen to prepare tumor vaccine. Tumor-bearing mice were divided into four groups and treated on day-5 and day-10 respectively. Group A was injected with NS, Group B with CTX (50 mg/kg), Group C with dendritic cells induced by astragalus polysaccharides and Group D with dendritic cells induced by tumor necrosis factor (TNF)-α. After 12 days of tumor-bearing, the animals were killed. The subcutaneous sarcoma, thymus and spleen were separated and weighted. The inhibitory rate, thymus index and spleen index were then calculated. ELISA assay was used to detect the levels of interleukin (IL)-4, interferon (IFN)-γ in serum of tumor bearing mice.   Results   The tumor inhibition rate was higher in astragalus polysaccharide group and cytokine group than that of CTX group (64.25%, 64.10% vs 35.11%). The thymus index was higher in astragalus polysaccharide group and cytokine group than that of CTX group (1.69 ± 0.26, 1.74 ± 0.38 vs 1.45 ± 0.22). The spleen index was higher in astragalus poly?saccharide group and cytokine group than that of CTX group (5.44 ± 0.76, 5.31 ± 0.81 vs 3.54 ± 0.52). The level of IL-4 was lower in astragalus polysaccharide group and cytokine group than that of CTX group (15.66±2.57, 14.72 ± 4.84 vs 23.95 ± 6.07). The level of IFN- γ was higher in astragalus polysaccharide group and cytokine group than that of CTX group (16.54 ±
3.71, 17.20 ± 2.03 vs 10.37 ± 2.19). All the differences were statistically significant (P<0.05).  Conclusion  Dendritic cell vaccine induced by astragalus polysaccharides can effectively inhibit tumor growth. Its mechanism may be associated with the promotion spleen index and thymus index of S180 tumor-bearing mice, the effective correction of Th1/Th2 imbalance induced by tumor, and the enhancement of antitumor immune responses.

Key words: astragalan, dendritic cells, interleukin-4, interferon type II, tumor inhibition rate, thymus index, spleen index, Th1/Th2