天津医药

天津医药 ›› 2017, Vol. 45 ›› Issue (7): 745-747.doi: 10.11958/20170353

• 临床研究 • 上一篇    下一篇

子宫内膜异位症患者腹腔液单个核细胞 IFN-γ 及 IL-10 mRNA 的表达及意义

李津鸿，王玉亮   

  1. 1 天津医科大学总医院医学检验科 （邮编 300052）； 2 天津市第一中心医院医学检验科
  • 收稿日期:2017-03-20 修回日期:2017-06-08 出版日期:2017-07-15 发布日期:2017-08-08
  • 通讯作者: 王玉亮 E-mail:wang_yu_l@163.com

Expressions and significance of IFN-γ and IL-10 mRNA in peritoneal fluid mononuclear cells of patients with endometriosis

LI Jin-hong,WANG Yu-liang   

  1. 1 Department of Clinical Laboratory, Tianjin Medical University General Hospital, Tianjin 300052, China;2 Department of Clinical Laboratory, Tianjin First Central Hospital
  • Received:2017-03-20 Revised:2017-06-08 Published:2017-07-15 Online:2017-08-08
  • Contact: WANG Yu-liang E-mail:wang_yu_l@163.com

PDF (PC)

3842

摘要： 目的 探讨子宫内膜异位症（EM）患者腹腔液单个核细胞（PFMC） γ 干扰素（IFN-γ）及白细胞介素（IL） - 10 mRNA 的表达水平及其意义。方法 选取经手术后病理证实为 EM 的患者 55 例作为 EM 组， 以非 EM 患者 52 例作为对照组。分离 PFMC， 应用 TaqMan 实时荧光逆转录聚合酶链反应（RT-PCR）测定 IFN-γ 及 IL-10 mRNA 的表达水平。结果 EM 组 PFMC IFN-γ mRNA 的表达水平低于对照组， 而 IL-10 mRNA 的表达水平明显高于对照组 （P<0.05）， EM 组 IFN-γ/IL-10 比值较对照组明显降低 （P<0.05）； Ⅰ~Ⅱ期 EM 患者 IFN-γ/IL-10 比值高于Ⅲ~Ⅳ期患者， 差异有统计学意义 （P<0.05）。结论 EM 患者 PFMC 中 IFN-γ/IL-10 失衡可能与 EM 的发生发展有关。

关键词: 子宫内膜异位症, 干扰素Ⅱ型, 白细胞介素 10, 单个核细胞, 腹腔液

Abstract: Objective To investigate the expression levels and significance of interferon-γ (IFN-γ), interleukin (IL)-10 mRNA in peritoneal fluid mononuclear cells (PFMC) in patients with endometriosis (EM). Methods A total of 55 patients with EM diagnosed histopathologically after operation were enrolled as EM group, and 52 patients without EM were enrolled as control group. The PFMC were isolated, and the expression levels of IFN-γ and IL-10 mRNA were determined by TaqMan real-time reverse transcriptase-polymerase chain reaction (RT-PCR). Results It was found that IFN-γ mRNA expression level was significantly decreased in EM group compared with that in control group (P<0.05), while IL-10 mRNA expression level was significantly increased in EM group compared with that in control group (P<0.05). The ratio of IFN-γ/IL-10 was significantly lower in EM group than that in control group (P<0.05). The ratio of IFN-γ/IL-10 was significantlyhigher in patients with stageⅠ-Ⅱ than that in patients with stage Ⅲ-Ⅳ (P<0.05). Conclusion The imbalance of IFN-γ/IL-10 in PFMC is associated with the pathogenesis of EM.

Key words: endometriosis, interferon type Ⅱ, interleukin 10, mononuclear cells, peritoneal fluid

中图分类号: