天津医药

天津医药 ›› 2014, Vol. 42 ›› Issue (12): 1193-1196.doi: 10.3969/j.issn.0253-9896.2014.12.012

• 实验研究 • 上一篇    下一篇

泽泻汤加味方对高盐高血压大鼠肾脏AQP-2表达的影响

景文莉1,王长志2,范洪亮3,张树峰3   

  1. 1. 天津医学高等专科学校
    2. 河北联合大学中医学院
    3. 承德医学院
  • 收稿日期:2014-03-06 修回日期:2014-07-22 出版日期:2014-12-15 发布日期:2014-12-15
  • 通讯作者: 景文莉 E-mail:jingwenli00@163.com
  • 基金资助:
    河北省科学技术支撑项目计划课题

Effect of Modified Zexie Decoction on Renal Aquaporin-2 Expression in Hypertension RatsInduced by High-Salt

JING Wenli 1, WANG Changzhi 2,FAN Hongliang 3, ZHANG Shufeng 3   

  1. 1. Chengde Medical University， Chengde 067000, China
    2. Hebei Union University College of Traditional Chinese Medicine
    3. Chengde Medical University， Chengde 067000, China;
  • Received:2014-03-06 Revised:2014-07-22 Published:2014-12-15 Online:2014-12-15
  • Contact: JING Wenli E-mail:jingwenli00@163.com

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摘要：

【摘要】 目的 探讨泽泻汤加味方对高盐高血压大鼠肾脏水通道蛋白（AQP）-2 表达的影响。 方法 以 8%高盐饲料喂养的方法制备高盐高血压动物模型 50 只， 造模成功后随机分为模型组， 西药组， 中药高、中、低组， 各 10 只； 另选 10 只普通饲料喂养大鼠为正常组。 中药高、中、低组分别给予泽泻汤加味方混悬液 16.2、10.8、5.4 g/(kg· d)；西药组给予缬沙坦氢氯噻嗪溶液 16.65 mg/(kg· d)； 模型组及正常组灌服等体积蒸馏水。 均按每天 1 mL/100 g 灌胃，灌胃 4 周。 于给药 1、4、7、14、28 d 时测定大鼠收缩压（SBP）， 并收集 24 h 尿量。 用免疫组织化学法和逆转录-聚合酶链反应（RT-PCR）分别检测肾脏 AQP-2 蛋白和 mRNA 的表达。 结果 SBP 由高到低依次是： 模型组＞中药中组和中药低组＞西药组和中药高组＞正常组， 尿量由高到低依次是： 西药组和中药高组＞中药中组和中药低组＞模型组＞正常组， 中药中组与中药低组间、西药组与中药高组间差异无统计学意义。 模型组肾脏 AQP-2 沿集合管内壁上皮细胞分布的棕黄色颗粒较正常组及中药高、中、低组明显颜色加深而浓厚， 分布面积更广泛。 除中药低组 AQP-2 mRNA 的表达水平与模型组差异无统计学意义外， 其余各组均低于模型组， 高于中药高组（均 P＜ 0.05）。 结论 泽泻汤加味方可通过抑制 AQP-2 的高表达使血压降低。

关键词: 高盐, 高血压, 肾脏, 水通道蛋白-2, 收缩压, 泽泻汤加味方

Abstract:

[Abstract] Objective To explore the effect of modified Zexie Decoction on renal aquaporin-2 (AQP2) expression in high-salt hypertensive rat. Methods Hypertensive rats model was established by feeding rat with 8% high salt. Rats (n= 50) were divided into model group, modern medicine group, traditional Chinese medicine groups of high, medium, low dose, with 10 rats in each group. The other 10 rats were fed with ordinary diet as normal group. Rats in traditional Chinese medi? cine of high, medium, low groups were given Zexie Decoction suspension of 16.2, 10.8 and 5.4 g/(kg· d) respectively; Rats in modern medicine group was given Valsartan hydrochlorothiazide 16.65 mg/(kg· d); the model group and normal group was ad? ministered with equal volume of distilled water. Animals were feed with medications at 1 mL/100 g by gavage for 4 weeks. On the 1 st , 4th , 7th , 14th and, 28th day of administration, we measured SBP and collected 24 h urine. We employed immunohis? tochemistry to detect renal AQP-2 protein expression level and RT - PCR to detect renal AQP -2 mRNA transcription level. Results The rank of SBP from high to low is: model group > traditional Chinese medicine medium and low dose groups > traditional Chinese medicine high dose group and western medicine group > normal group. The rank of urine volume from high to low is: Western medicine group and traditional Chinese medicine high dose group > traditional Chinese medicine me? dium and low dose group > normal group, the difference was not statistically significant between traditional Chinese medi? cine medium and low dose group , or between western medicine group and traditional Chinese medicine high dose group. The renal AQP - 2 in epithelial cells along the collecting duct wall of rats in model group show brown particles which are darker and wider distributed than those in normal group and traditional Chinese medicine of high, medium, low dose groups. RT - PCR results show that AQP-2 mRNA expression is highest in rats of model group and lowest in rats of traditional Chi? nese medicine high dose group (P < 0.05). No statistical significance of AQP mRNA level was found between traditional Chi? nese medicine low group and model group (P < 0.05). Conclusion Modified Zexie Decoction can lower blood pres? sure by inhibiting the expression of AQP-2

Key words: high salt, hypertension, kidney, Aquaporin - 2., SBP, modified zexie decoction