天津医药

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地塞米松对大鼠骨密度及体成分的影响

刘思瑶,张劼,黄松,冼晶,罗佐杰,梁敏   

  1. 广西医科大学第一附属医院内分泌科
  • 收稿日期:2013-09-03 修回日期:2013-09-21 出版日期:2014-02-15 发布日期:2014-02-15
  • 通讯作者: 刘思瑶

Effects of dexamethasone on bone mineral density and body composition in rats

  • Received:2013-09-03 Revised:2013-09-21 Published:2014-02-15 Online:2014-02-15

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摘要： 目的 探讨不同剂量地塞米松对SD大鼠骨密度（BMD）和体成分的影响。方法 3月龄SD雌性大鼠40只，按地塞米松的剂量分为对照组（生理盐水），低剂量组（1 mg/kg），中剂量组（2.5 mg/kg），高剂量组（5 mg/kg），每组10只，每周2次肌内注射。给药前、给药4周后应用DEXA骨密度仪测定大鼠全身BMD和体成分。结果 对照组体质量随时间逐渐增加，干预1～4周后不同剂量地塞米松干预组体质量均较对照组下降（P＜0.01）。干预4周后，对照组总BMD、股骨BMD、总BMC及全身、躯干、腿部脂肪含量均较干预前增加（P＜0.01），全身、躯干肌肉含量均较干预前下降（P＜0.01）；低剂量组、中剂量组大鼠总骨矿含量（BMC）、股骨BMD、腿部肌肉含量、腿部脂肪含量均低于对照组（P＜0.05）；低剂量组、中剂量组股骨BMD、腿部脂肪含量均低于高剂量组（P＜0.05）。结论 每周2次连续4周肌内注射地塞米松，1mg/kg和2.5 mg/kg较5 mg/kg的剂量对SD大鼠股骨BMD及腿部成分的影响更大，可降低SD大鼠股骨BMD、腿部脂肪含量。

关键词: 地塞米松, 骨密度, 体成分, 大鼠, Sprague-Dawley

Abstract: Objective To investigate the effects of different doses of dexamethasone (Dex) on bone mineral density (BMD) and body composition in Sprague-Dawley (SD) rats. Methods Forty female 3-month-old SD rats were divided into four groups by the doses of Dex, control group (Cont, saline), low dose group (LDG, 1mg/kg ), medium dose group (MDG, 2.5mg/kg) and high dose group (HDG, 5mg/kg). Ten rats for each group, injected intramuscularly twice a week. The BMD and body composition were measured at the beginning and 4 weeks later by DEXA densitometer. Results The body weight of Cont was increased with time, the body weight of different doses of Dex intervention groups were decreased after 1 to 4 weeks intervention when compared with the Cont. After 4 weeks intervention, the total BMD, femur BMD, total BMC, total fat mass, trunk fat mass, leg fat mass of Cont were increased while the total lean mass, trunk lean mass of Cont were decreased when compared with itself before intervention. The total BMC, femur BMD, leg lean mass, leg fat mass of LDG and MDG were lower than the cont (P＜0.05). The femur BMD, leg fat mass of LDG and MDG were lower than the HDG(P＜0.05). Conclusion The doses of 1mg/kg and 2.5mg/kg Dex have greater influence than the dose of 5 mg/kg Dex on the femur BMD and the leg composition of SD rats by intramuscular injections twice a week for 4 weeks, and can lead the femur BMD and leg fat mass decreased.

Key words: Dexamethasone, Bone mineral density, Body composition, rats, Sprague-Dawley