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荔枝核总黄酮改善BDL大鼠胆汁淤积症状的研究

成秋宸,赵永忠,肖绪华,刘瑞彪,黄大健,李胜联,徐庆   

  1. 桂林医学院附属医院
  • 收稿日期:2013-08-12 修回日期:2013-10-27 出版日期:2014-03-15 发布日期:2014-03-15
  • 通讯作者: 成秋宸

A experimental study of total flavone from Litchi chinensis Sonn improve the symptoms of cholestatasis BDL rats

  • Received:2013-08-12 Revised:2013-10-27 Published:2014-03-15 Online:2014-03-15

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摘要： 目的 观察荔枝核总黄酮对胆总管结扎肝纤维化（bile duct ligation，BDL）大鼠肝功能的改善及对胶原蛋白Ⅰ，3及p16蛋白的影响。方法 以胆总管结扎法造模，40只SD大鼠随机分为胆总管结扎组（BDL组），荔枝核总黄酮组（TFL组），水飞蓟宾组（SIL组），以假手术组（SO组）为阴性对照。四周后处死大鼠，取血清检测各组大鼠血清氧化物歧化酶（SOD）、丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、直接胆红素(BILD2)、总胆红素(BILT2)，同时，取肝组织行HE、Masson染色，western blot测定各组肝组织的PCⅠ、PC3、p16的表达。结果 与BDL组比较，TFL组血清SOD含量明显升高(P<0.05),ALT，AST，BILD2，BILT2水平明显降低(P<0.05),TFL组肝纤维化程度明显减轻(P<0.05),TFL组PCⅠ、PC3、p16蛋白含量明显减少(P<0.05)。结论 TFL有明显抗氧化，改善胆汁淤积性肝纤维化大鼠肝功能，减轻肝组织纤维化；其机制可能与抑制肝组织p16、PC3、PCⅠ蛋白表达有关。

关键词: 荔枝核总黄酮, 胆汁淤积性肝纤维化, Ⅰ型胶原蛋白, PC3, p16

Abstract: Objective Observed total flavone from Litchi chinensis Sonn on liver function improvement and collagen Ⅰ, 3 and p16 in bile duct ligation liver fibrosis (bile duct ligation, BDL) rats.Methods The rats model is the rats after ligation of the common bile duct，forty SD rats was randomized into the common bile duct ligation (BDL group), Litchi flavonoids group (TFL group), silibinin group (SIL group),to the control group (SO group) as a negative control. After four weeks,serum detection of serum superoxide dismutase(SOD),alanine aminotransferase(ALT),aspartate aminotransferase (AST), direct bilirubin (BILD2), total bilirubin (BILT2) , creatinine (CREJ2), blood urea nitrogen (UREAL) were measured, and the pathological observation was also performed on legt liver lobes with HE, Masson staining, western blot were measured in liver tissue PC3, PC Ⅰ, p16 expression.Results The serum SOD in TFL was significantly higher than that in the BDL group (P <0.05), The serum ALT, AST, BILD2, BILT2 levels were significantly lower than that in the BDL group(P <0.05), TFL can significantly improve the degree of hepatic fibrosis in rats (P <0.05 ), TFL can inhibit p16, PC3, PC Ⅰprotein expression (P <0.05).Conclusion TFL group has a significant improvement in cholestasis and liver fibrosis mitigate, significantly inhibited cholestatic liver fibrosis in rat liver p16, PC3, PC Ⅰ protein.

Key words: flavone from Litchi chinensis Sonn, Cholestatic liver fibrosis, PC3, PC Ⅰ, p16