天津医药

• 细胞与分子生物学 • 上一篇    下一篇

结直肠息肉、结直肠腺瘤和结直肠癌患者外周血中Th17和Tc17细胞的变化

王建升,张立志,帖彦清,吕元鹏,宋津晓   

  1. 河北省人民医院
  • 收稿日期:2013-07-18 修回日期:2013-12-11 出版日期:2014-04-15 发布日期:2014-04-15
  • 通讯作者: 王建升

The Changes of Circulating Th17and Tc17Cells in Patients with Colorectal Polyp,Adenoma and Cancer

  • Received:2013-07-18 Revised:2013-12-11 Published:2014-04-15 Online:2014-04-15
  • Contact: Jians-heng WANG

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摘要： [摘 要] 目的 检测结直肠息肉、结直肠腺瘤和结直肠癌患者外周血中Th17与Tc17细胞比例的变化，并分析二者的相关性。方法 收集河北省人民医院结直肠息肉17例、结直肠腺瘤35例和结直肠癌44例，另取18名健康志愿者为对照。流式细胞术检测患者和健康对照外周血中Th17和T c17细胞比例；ELISA检测血清中IL-17A和IL-23水平。结果 结直肠息肉、结直肠腺瘤和结直肠癌患者外周血中Th17和Tc17细胞比例及因子IL-17A和IL-23水平均高于对照组 (p<0.05)。结直肠癌组Th17和Tc17细胞比例及因子IL-17A和IL-23水平均高于结直肠息肉组或结直肠腺瘤组 (p<0.05)；Tc17细胞比例在结直肠腺瘤组高于结直肠息肉组 (p<0.05)，但Th17细胞及因子IL-17A和IL-23水平在两组间并无统计学意义 (p>0.05)。相关性分析表明Th17和Tc17细胞呈正相关 (r = 0.594, p < 0.001)。结论 在结直肠息肉或结直肠腺瘤向结直肠癌发展中，外周血Th17和Tc17细胞比例都明显增多且二者呈正相关，表明两类细胞都参与了结直肠癌发生发展的免疫病理过程。

关键词: 结直肠息肉, 结直肠腺瘤, 结直肠癌, Th17细胞, Tc17细胞, 细胞因子

Abstract:

[Abstract] Objective To compare ratio of circulating Th17over Tc17cells in patients with colorectal polyp (CRP), colorectal adenoma (CRA) and colorectal cancer (CRC)，and analyse their relationship.Methods The ratio of circulating Th17over Tc17cells in93patients（17CRP,34CRA and42CRC）and17healthy donors were examined by flow cytometry; Serum levels of IL-17A and IL-23were tested by ELISA.Results The ratio of circulating Th17over Tc17cells and the se? rum levels of IL-17A and IL-23were the lowest in healthy control groups, medium in CRP and CRA groups and the highest in CRC groups (P<0.05). The proportions of Tc17cells in CRA groups were significantly higher compared to those in CRP groups，but no statistical significance of proportion of circulating Th17cells as well as serum levels of IL-17A and IL-23be? tween CRA and CRP groups was observed (P>0.05). Further analysis showed that there was a positive correlation between proportions of circulating Th17and Tc17cells (rs=0.594, P<0.001). Conclusion The proportions of circulating Th17and Tc17cells were positively related and the ratio of Th17over Tc17was markedly increased from CRP group or CRA group to CRC group.

Key words: Colorectal polyp, Colorectal adenoma, Colorectal cancer, Th17 cells, Tc17 cells, Cytokines