天津医药

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1，25-二羟维生素D3减轻严重烫伤小鼠应激反应的作用

刘曼1,张明谏2,李小兵2,刘光晶3,王玉亮4,张建1,刘宁2   

  1. 1. 天津医科大学一中心临床学院
    2. 天津市第一中心医院烧伤整形科
    3. 天津市第一中心医院烧伤科
    4. 天津市第一中心医院
  • 收稿日期:2013-10-15 修回日期:2014-01-15 出版日期:2014-05-15 发布日期:2014-05-15
  • 通讯作者: 张明谏

Effect of 1,25-dihydroxyvitamin D3 on alleviating the stress response in severely scalded mice

  • Received:2013-10-15 Revised:2014-01-15 Published:2014-05-15 Online:2014-05-15
  • Contact: ZHNG Ming-Jian

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摘要： 目的：探讨1，25-二羟维生素D3对严重烫伤小鼠应激状态下胰岛素抵抗和炎症反应的影响。方法： 130只小鼠随机分为：健康组10只，实验组Ⅰ、实验组Ⅱ、对照组各40只。实验组Ⅰ、实验组Ⅱ和对照组小鼠制成30%全身体表面积（TBSA）Ⅲ°烫伤模型。每隔1日晨8时，实验组Ⅰ小鼠1，25-(0H)2VitD3 1μg?kg-1+0.6mL花生油灌胃；实验组Ⅱ小鼠1，25-(0H)2VitD3 4μg?kg-1+0.6mL花生油灌胃；对照组小鼠0.6mL花生油灌胃。分别于伤后1、3、7、14 天测空腹血糖（FBG）、空腹胰岛素（FIns）、血清TNF-α浓度和创面组织NF-κB阳性率。结果：（1）实验组Ⅰ、实验组Ⅱ和对照组各时相点胰岛素抵抗指数（HOMA-IR）、血清TNF-α含量和创面组织NF-κB表达阳性率均数均高于健康组水平。（2）实验组Ⅰ和实验组Ⅱ小鼠伤后同一时相点胰岛素抵抗指数（HOMA-IR）均数均低于对照组水平，且实验组Ⅱ低于实验组Ⅰ（P<0.05）；同一组内不同时相点实验小鼠伤后第3天HOMA-IR均数最高，以后逐渐降低（P<0.05）。（3）实验组Ⅰ和实验组Ⅱ小鼠伤后同一时相点血清TNF-α含量、创面组织NF-κB表达阳性率均数均低于对照组水平，且实验组Ⅱ低于实验组Ⅰ（P<0.05）；同一组内不同时相点血清TNF-α含量、创面组织NF-κB表达阳性率均数均逐渐降低（P<0.05）。结论：1，25-二羟维生素D3能够减轻严重烫伤小鼠应激状态下胰岛素抵抗作用和炎症反应。

关键词: 25-二羟维生素D3, 小鼠, 烫伤, 胰岛素抵抗, 肿瘤坏因子-α, 核因子-κB

Abstract: Objective To explore the effect of 1,25-dihydroxyvitamin D3 on insulin resistance and inflammatory response in mice with severe burns under stress. Methods 130 mice were randomly divided into: the healthy group (n = 10), the experimental group I (n = 40), the experimental group II (n = 40), the control group (n = 40). Each mouse was scalded in the experimental group I ,the experimental group II and control group to 30% of the total body surface area (TBSA) III °. Mice in experimental group I received 1，25-(0H)2VitD3 1μg?kg-1 with 0.6 mL peanut oil by gavaging at 8 o'clock every other day.At the same time and by the same way, mice in experimental group II received 1，25-(0H)2VitD3 4μg?kg-1 with 0.6 mL peanut oil，and mice in control group received only 0.6mL peanut oil. FBG, FIns, serum TNF-α concentrations and wound tissue NF-κB positive rate were determined respectively on day 1, day3, day7 and day 14 after burn . Results (1) At each time point of the experimental groupⅠ, the experimental groupⅡand control group, the means of HOMA-IR , Serum TNF-α concentrations and wound tissue NF-κB positive rate were higher than those in healthy group. (2) The means of HOMA-IR of scalded mice in experimental group Ⅰand experimental groupⅡ were lower than those in control group at the same time, and the means of experimental group Ⅱ were lower than those in the experimental group Ⅰ (P <0.05); at the different time points ,the means of HOMA-IR of scalded mice in same group were the highest at day 3, and then gradually decreased (P <0.05). (3) The means of Serum TNF-α concentrations and wound tissue NF-κB positive rate of scalded mice in experimental group Ⅰand experimental groupⅡ were lower than those in control group at the same time, and the means of experimental group Ⅱ were lower than those in the experimental group Ⅰ (P <0.05); at the different time points ,the means of Serum TNF-α concentrations and wound tissue NF-κB positive rate of scalded mice in same group were gradually decreased (P <0.05). Conclusion 1,25-dihydroxyvitamin D3 can reduce insulin resistance and inflammatory response in mice with severe burns under stress.

Key words: 25-dihydroxyvitamin D3, mice, burns, insulin resistance, tumor necrosis factor-alpha, nuclear factor-kappa B