天津医药

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MicroRNA-1对大鼠心肌细胞凋亡的影响

王霁翔1,丛洪良2   

  1. 1. 天津市胸科医院
    2. 天津胸科医院
  • 收稿日期:2013-08-26 修回日期:2013-12-30 出版日期:2014-05-15 发布日期:2014-05-15
  • 通讯作者: 王霁翔

Apoptosis effects of microRNA-1 on cardiomyocytes

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  • Received:2013-08-26 Revised:2013-12-30 Published:2014-05-15 Online:2014-05-15

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摘要： 摘要 目的 探讨microRNA-1(miR-1) 对大鼠心肌细胞凋亡的影响。方法 应用转染的方法使培养的H9C2大鼠心肌细胞高表达miR-1（miR-1组），荧光定量PCR的方法确定miR-1上调后，采用MTT法和流式细胞术检测各组细胞活力和凋亡情况、用荧光定量PCR和Western Blot的方法检测凋亡抑制因子Bcl-2的mRNA和蛋白表达情况。正常细胞和转染随机合成的miRNA阴性对照片段分别为正常对照组和阴性对照组。结果 与正常对照组相比，阴性对照组各个指标的变化均没有统计学差异。miR-1组的miR-1 mRNA表达水平显著升高，细胞活力下降，凋亡率增加，Bcl-2的mRNA和蛋白表达水平均明显降低。 结论 转染的miR-1 mimic能够上调细胞内的miR-1mRNA表达水平，抑制心肌细胞增殖，促进细胞凋亡。

关键词: 心肌细胞, 细胞凋亡, microRNA , microRNA-1 (miR-1)

Abstract: Abstract: Objective To investigate the effect of microRNA-1 (miR-1) on H9C2 cardiomyocytes. Methods MiR-1 mimic was transfected into the cultured H9C2 cell line was defined as miR-1 group. Cells transfected with random miRNA fragement was negative control group. Normal control cells had no treatment. The MTT and flow cytometry method were used to test cell vitality and apoptosis rate,while the mRNA and protein expression level of Bcl-2 were detected by real time PCR and western blot methods. Results Compared with the normal control group, negative control cells had no changes. The application of miR-1 mimic can significantly increase the miR-1 level and apoptosis rate, reduce cell vitality and Bcl-2 expression level. Conclusion miR-1 mimic can up-regulate miR-1 level, inhbit proliferation and induce cardiomyocyte apoptosis .

Key words: cardiomyocyte, cell apoptosis, microRNA, microRNA-1 (miR-1)