天津医药

• 细胞与分子生物学 • 上一篇    下一篇

HBV感染的肝癌患者外周血中调节性T细胞的水平分析

刘辉1,李建宇2,王凤梅3,王鹏1,李成龙1,骆莹1,朱争艳3   

  1. 1. 天津市第三中心医院肝胆疾病研究所，天津市人工细胞重点实验室
    2. 武警后勤学院
    3. 天津市第三中心医院
  • 收稿日期:2013-12-16 修回日期:2014-02-13 出版日期:2014-06-15 发布日期:2014-06-15
  • 通讯作者: 刘辉

Analysis of CD4+CD25+CD127low regulatory T cells in the peripheral blood of hepatocellular carcinoma patients with HBV infection

  • Received:2013-12-16 Revised:2014-02-13 Published:2014-06-15 Online:2014-06-15
  • Contact: LIU Hui

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摘要： 目的 观察乙肝病毒(HBV)感染的肝癌患者外周血中CD4+CD25+CD127low调节性T细胞(Tregs)的水平，分析其与HBV感染的相关性。方法 采集41例感染HBV的肝癌患者、34例未感染HBV的肝癌患者及29例正常健康受试者的外周抗凝血，利用流式细胞术(FCM)检测各组受试者外周血中CD4+CD25+CD127lowTregs水平，通过荧光定量聚合酶链反应(FQ-PCR)检测感染HBV的肝癌患者血清中HBV DNA载量，分析Tregs水平与HBV DNA载量的相关性。结果 感染HBV的肝癌患者外周血中CD4+CD25+CD127lowTregs占CD4+T细胞的比率显著高于未感染HBV的肝癌患者[(8.24±2.20)%比(7.06±2.46)%, P<0.05]和正常健康受试者[(8.24±2.20)%比(6.51±1.99)%, P<0.01]，未感染HBV的肝癌患者外周血中CD4+CD25+CD127lowTregs占CD4+T细胞的比率亦高于正常健康受试者[(7.06±2.46)%比(6.51±1.99)%, P<0.05]；随HBV DNA载量的增高，感染HBV的肝癌患者外周血中CD4+CD25+CD127lowTregs占CD4+T细胞的比率升高，CD4+CD25+CD127lowTregs水平与HBV DNA载量(取对数转换)呈显著正相关(r=0.350，P<0.05)。结论 肝癌患者外周血中Tregs的水平异常增高，且与HBV DNA复制密切相关，这可能是肝癌细胞免疫逃逸的重要原因。

关键词: 肝癌, 乙肝病毒, 调节性T细胞, 免疫功能, CD127

Abstract: Objective To investigate the level of CD4+CD25+CD127low regulatory T cells in the peripheral blood of hepatocellular carcinoma (HCC) patients with HBV infection and to explore the relationship between these cells and HBV infection. Methods Peripheral blood mononuclear cells were isolated from 41 HCC patients infected with HBV, 34 HCC patients without HBV infection and 29 healthy persons. These mononuclear cells were labeled with the monoclonal antibodies of CD4, CD25 and CD127, and then they were determined by flow cytometry (FCM) to analyze the level of Tregs. Moreover, the load of HBV DNA in the blood serum of HCC patients with HBV infection was detected by fluorescent quantitative polymerase chain reaction (FQ-PCR). Results The percentage of CD4+CD25+CD127lowTregs in CD4+T cells in the HCC patients with HBV infection was higher than that in the HCC patients without HBV infection [(8.24±2.20)% vs (7.06±2.46)%, P<0.05] and that in the healthy persons [(8.24±2.20)% vs (6.51±1.99)%, P<0.01]. Furthermore, the percentage of CD4+CD25+CD127lowTregs in CD4+T cells in the HCC patients without HBV infection was higher than that in the healthy persons [(7.06±2.46)% vs (6.51±1.99)%, P<0.05]. In addition, the level of CD4+CD25+CD127lowTregs in the HCC patients with HBV infection was gradually increased following the increase of HBV DNA load and there was a positive correlation between the level of CD4+CD25+CD127lowTregs and the load of HBV DNA (r=0.350，P<0.05). Conclusion The level of CD4+CD25+CD127low Tregs in HCC patients is increased remarkably and associated with the copy of HBV DNA, which may be an important mechanism of immune evasion in HCC.

Key words: Hepatocellular Carcinoma (HCC), Hepatitis B Virus (HBV), Regulatory T Cells (Tregs), Immunologic Function, CD127