天津医药

• 细胞与分子生物学 • 上一篇    下一篇

强力霉素对胃癌细胞增殖及Notch1、 MMP-9表达的影响

许敏,李红   

  1. 辽宁医学院
  • 收稿日期:2013-11-08 修回日期:2014-02-17 出版日期:2014-06-15 发布日期:2014-06-15
  • 通讯作者: 许敏

Doxycycline on gastric cancer cell proliferation and Notch1,MMP-9 expression

  1. 1. 辽宁医学院生物化学教研室
    2.
  • Received:2013-11-08 Revised:2014-02-17 Published:2014-06-15 Online:2014-06-15
  • Contact: 李红

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摘要： 【摘要】目的 研究强力霉素对胃癌细胞BGC-823细胞的增殖及 Notch1、MMP-9 蛋白表达的影响，为胃癌的治疗提供新的抗肿瘤药物。方法 分别以 0、5、10、20、40 μg/mL 终浓度的强力霉素作用于人胃癌细胞系 BGC-823，运用 MTT 法检测细胞增殖程度；流式细胞仪检测细胞周期分布的影响； Western Blot 检测 Notch1、MMP-9 蛋白水平的表达。结果 强力霉素能够抑制人胃癌细胞BGC-823细胞的增殖，且与药物的剂量及作用时间呈正相关（P＜0.01）；强力霉素能够改变胃癌细胞BGC-823周期的分布，随着浓度的增加，S 期所占比例降低（P＜0.01）；并且随着浓度的增加，Notch1 的表达增强，MMP-9 的表达降低（P＜0.01）。结论 强力霉素能够抑制胃癌细胞BGC-823的增殖，Notch1上调为可能的机制之一

关键词: 胃癌细胞, 强力霉素, Notch1, MMP-9, 细胞增殖, 细胞周期

Abstract: 【Abstract】: Objective: Doxycycline on gastric cancer cell line BGC-823 proliferation and Notch1,MMP-9 expression, in order to provide a new new anticancer drugs. Method: Respectively 0, 5, 10, 20, 40 μg/mL final concentration of doxycycline effects on human gastric cancer cell line BGC-823.Cell proliferation was detected was detected by MTT degree; flow cytometry cell cycle distribution; Western Blot detection Notch1, MMP-9 protein expression. Results: Doxycycline can inhibit the proliferation of human gastric cancer cells line BGC-823, and the effect of drug dose and time-dependent（P＜0.01）; doxycycline alter gastric cancer cell line BGC-823 cycle distribution, with increasing concentration, S the proportion of lower phase（P＜0.01）; as the concentration increase, Notch1 expression enhancement, MMP-9 expression decreased （P＜0.01）. Conclusion: Doxycyclinecan inhibit gastric cancer cell line BGC-823 proliferation, Notch1 up as one of the possible mechanisms.

Key words: gastric cancer cells, doxycycline, Notch1, MMP-9, cell proliferation, cell cycle