天津医药

天津医药 ›› 2017, Vol. 45 ›› Issue (7): 699-703.doi: 10.11958/20170481

• 实验研究 • 上一篇    下一篇

茶黄素对胸腺放射性损伤的防护作用

韩晓丹，张俊伶，薛晓蕾，赵玉，樊赛军   

  1. 北京协和医学院中国医学科学院放射医学研究所， 天津市放射医学与分子核医学重点实验室 （邮编 300192）
  • 收稿日期:2017-04-18 修回日期:2017-06-12 出版日期:2017-07-15 发布日期:2017-08-08
  • 通讯作者: 张俊伶， 樊赛军 E-mail:hanxiaodan1202@163.com
  • 基金资助:
    国家自然科学基金资助项目 （81402633， 81572969， 81402541）； 科技部科研院所技术发展研究专项 （2014EG150134）； 中国医学科学院医学与健康创新工程重大项目（2016-I2M-1-017）； 天津科技支撑计划项目（14ZCZDSY00001）； 天津市自然科学基金青年项目（16JCQNJC13600）

Protective effects of theaflavins on radiation injury of thymus

HAN Xiao-dan,ZHANG Jun-ling,XUE Xiao-lei,ZHAO Yu,FAN Sai-jun   

  1. Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Science and Peking Union Medical College, Tianjin 300192, China
  • Received:2017-04-18 Revised:2017-06-12 Published:2017-07-15 Online:2017-08-08

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摘要： 目的 探讨茶黄素对小鼠胸腺放射性损伤的防护作用。方法 25 只 C57BL/6 小鼠随机分为对照组、 照射组、 照射+25 mg/kg 茶黄素组、 照射+50 mg/kg 茶黄素组、 照射+100 mg/kg 茶黄素组。对照组小鼠给予 0 Gy 照射， 其余小鼠接受 4 Gy 全身照射。照射后第 14 天取小鼠胸腺检测胸腺指数、 胸腺细胞总数， 以确定茶黄素的最佳给药剂量。另取 32 只 C57BL/6 小鼠， 根据最佳给药剂量将其随机分为对照组、 茶黄素组、 照射组、 照射+茶黄素组， 照射组和照射+茶黄素组小鼠接受 4 Gy 的全身照射。照射后第 14 天检测胸腺组织形态、 CD4CD8 T 细胞亚群和胸腺细胞活性氧 （ROS） 水平。结果 受辐射小鼠胸腺有放射性损伤， 表现为胸腺指数下降， 胸腺细胞总数减少， 胸腺组织形态改变， 胸腺 T 细胞亚群改变及胸腺细胞 ROS 水平升高。与照射组相比， 照射+50 mg/kg 茶黄素组小鼠胸腺指数和胸腺细胞总数升高（P＜0.05）， 且照射+50 mg/kg 茶黄素组小鼠胸腺细胞总数高于照射+25 mg/kg 茶黄素组（P＜0.05）。与照射组相比， 照射+茶黄素组胸腺病理改变改善， 胸腺 CD4+CD8-、 CD4+CD8+细胞比例升高， 而 CD4-CD8-细胞比例下降 （P＜0.05）， 胸腺细胞内 ROS 水平降低 （P＜0.05）。结论 茶黄素对 4 Gy 全身照射诱导的小鼠胸腺放射性损伤具有一定的保护作用。

关键词: 胸腺, 辐射损伤, 活性氧, 小鼠, 近交 C57BL, 茶黄素, 免疫功能

Abstract: Objective To investigate the protective effect of theaflavins on thymus injury caused by total body irradiation (TBI). Methods Twenty- five C57BL/6 mice were randomly divided into 5 groups: control group, 4 Gy TBI group, 4 Gy TBI + 25 mg/kg theaflavins group, 4 Gy TBI + 50 mg/kg theaflavins group and 4 Gy TBI + 100 mg/kg theaflavins group. Thymus index and total number of thymocytes were detected at the 14th d post-irradiation to determine the optimal dose of theaflavins. According to this optimal dose, 32 C57BL/6 mice were randomly divided into 4 groups: control group,theaflavins group, 4 Gy TBI group and 4 Gy TBI + theaflavins group. Thymus histomorphology, CD4CD8 T cell subsets, and reactive oxygen species (ROS) in thymocytes were examined at the 14th d post-irradiation. Results The irradiated thymus exhibited decreased thymus index and total number of thymocytes (P＜0.05), aberrant histomorphology and T cell subsets (P＜0.05), and increased ROS level in thymocytes (P＜0.05). Compared with 4 Gy TBI group, the thymus index and total number of thymocytes were significantly increased in 4 Gy TBI + 50 mg/kg theaflavins group (P＜0.05). The total number of thymocytes was significantly higher in 4 Gy TBI + 50 mg/kg theaflavins group than that in 4 Gy TBI + 25 mg/kg theaflavins group (P＜0.05). Therefore, 50 mg/kg theaflavins was chosen as the optimal dose for subsequent experiments. Moreover, the aberrant histomorphology of irradiated thymus was alleviated by theaflavins. A decline in the percentage of CD4-CD8- T cellsand an elevation of CD4+CD8- and CD4+CD8+ T cells were found in irradiated mice administered with theaflavins (P＜0.05).Compared with 4 Gy TBI group, the ROS level was significantly decreased in 4 Gy TBI + theaflavins group (P＜0.05).Conclusion Theaflavins exhibits a protective effect on radiation-induced thymus injury.

Key words: thymus gland, radiation injuries, reactive oxygen species, mice, inbred C57BL, theaflavins, immune function

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