天津医药

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改良冠状动脉介入技术建立猪冠状动脉微栓塞动物模型

马国添1,曾志羽1,2,吴海2,3,罗晨源2,4,林士云1,2   

  1. 1. 广西医科大学第一附属医院老年心内科
    2.
    3. 广西医科大学第一附属医院心血管内科
    4. 广西医科大学病理生理学教研室
  • 收稿日期:2013-10-08 修回日期:2014-02-17 出版日期:2014-06-15 发布日期:2014-06-15
  • 通讯作者: 马国添

The Improved Technology of Inducing Coronary Microembolization Animal Model of Pigs by Coronary Intervention

  • Received:2013-10-08 Revised:2014-02-17 Published:2014-06-15 Online:2014-06-15
  • Contact: Guotian Ma

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摘要： 目的 探讨简便又经济的建立小型猪冠状动脉微栓塞（CME）模型的技术。方法 小型猪11只，随机分为对照组5只，CME组6只。采用桡动脉穿刺针穿刺股动脉，5F造影管及1.8F微导管往CME组左前降支注入栓塞微球。检测栓塞前及栓塞6h后血清脑利钠肽及心肌肌钙蛋白I(cTnI)水平。栓塞6h后取心尖部心肌光镜下观察。结果 CME组栓塞6h后血清脑利钠肽及cTnI水平较对照组明显增高（P<0. 001；P<0.001）。光镜下CME组心肌可见栓塞微球、微梗死、炎症细胞浸润。结论 利用新的手术器材可以成功建立小型猪CME模型，该技术简便、经济、实用，值得推广。

关键词: 冠状动脉微栓塞, 血管内操作, 动物模型, 小型猪

Abstract: Abstract Objective: To develop an animal model of miniature pigs with coronary microembolization (CME) by easy and economical technology. Methods: A total of 11 miniature pigs were divided into control group (n=5) and CME group (n=6). Femoral artery puncture was made with a 21 gauge needle for transradial procedures. Microspheres were injected into the left anterior descending artery of the CME group by 5F coronary diagnostic catheter and 1.8F coronary micro-guide catheter. Serum concentrations of brain natriuretic peptide (BNP) and cardiac troponin I (cTnI) were evaluated just before CME and 6h after CME. Apical myocardial pathological lesions 6h after CME were evaluated by optical microscope. Results: All the miniature pigs of the control group survived, but one died in the CME group. 5F coronary diagnostic catheter and 1.8F coronary micro-guide catheter successfully arrived at the designated location. Before CME, the serum concentrations of BNP (ng/L：143.00±13.51 vs 134.00±15.57) and cTnI (μg/L：0.39±0.09 vs 0.38±0.10) showed no significant differences between these two groups (t values are 0.976 and 0.294 respectively，both P＞0.05). After CME, the serum concentrations of BNP (561.00±80.65) and cTnI (2.75±0.58) much higher than those (BNP 139.00±13.87; cTnI 0.54±0.14 ) of the control group (t values are 11.530 and 8.337 respectively，both P＜0.001). In CME group, microspheres, micro-infarction and inflammatory cell infiltration were found under an optical microscope. Conclusion: The use of new surgical consumables can successfully develop an animal model of miniature pigs with CME, and the technology is simple, economical, practical and worth promoting.

Key words: coronary microembolization, endovascular procedures, animal model, swine, miniature