天津医药

天津医药 ›› 2016, Vol. 44 ›› Issue (5): 586-588.doi: 10.11958/20150202

• 实验研究 • 上一篇    下一篇

T2DM 小型猪模型骨骼肌、 肝脏及胰腺组织蛋白激酶 B 表达及磷酸化的变化

于健 1， 邹迪莎 2， 叶瑶 2， 周素娴 1， 郑天鹏 1， 杨帆 1   

  1. 1桂林医学院附属医院内分泌科 （邮编541001）； 2桂林医学院研究生院
  • 收稿日期:2015-10-12 修回日期:2015-12-29 出版日期:2016-05-15 发布日期:2016-05-18
  • 作者简介:1桂林医学院附属医院内分泌科 （邮编541001）； 2桂林医学院研究生院
  • 基金资助:
    广西科学研究与技术开发计划项目课题（桂科攻 1347003-5）； 桂林市科学研究与技术开发计划项目课题（20150126-1-1）； 广西壮族自治区卫生厅科研课题 （Z2014301）

Changes of protein kinase B and phosphorylation expression in the skeletal muscle, liver and pancreas of miniature pig model with type 2 diabetes mellitus model

YU Jian1, ZOU Disha2, YE Yao2, ZHOU Suxian1, ZHENG Tianpeng1, YANG Fan1   

  1. 1 Department of Endocrinology, the Affiliated Hospital of Guilin Medical College, Guangxi 541001, China; 2 Graduate School of Guilin Medical College
  • Received:2015-10-12 Revised:2015-12-29 Published:2016-05-15 Online:2016-05-18

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摘要： 摘要： 目的 研究高脂高糖饮食联合链脲佐菌素 （STZ） 构建巴马小型猪 2 型糖尿病 （T2DM） 模型的可行性， 并观 察小型猪模型骨骼肌、 肝脏及胰腺组织蛋白激酶 B（PKB）表达及磷酸化变化。方法 将健康雄性巴马小型猪 10 只 随机分为 2 组： 对照组 5 只， 喂养普通饲料； 糖尿病模型组 5 只， 高脂高糖饮食联合 STZ 构建巴马小型猪 T2DM 模 型。检测 2 组小型猪空腹血糖、 空腹胰岛素水平， 并计算胰岛素抵抗指数 （HOMA-IR）。采用 Western blot 法检测骨 骼肌、 肝脏及胰腺组织中 PKB 蛋白表达及磷酸化水平。结果 （1） 高脂高糖饲料喂养 10 个月后， 与对照组比较， 模 型组体质量、 空腹血糖、 空腹胰岛素水平及 HOMA-IR 明显升高 （P＜0.01）。（2） 与对照组相比， 模型组 STZ 给药后空 腹血糖水平进一步升高， 空腹胰岛素水平明显下降 （P＜0.01）。（3） 与对照组比较， 模型组骨骼肌、 肝脏及胰腺组织中 PKB 蛋白表达及磷酸化水平明显降低 （P＜0.05）。结论 高脂高糖饮食联合 STZ 多次腹腔注射可以成功构建 T2DM 小型猪模型， 模型组小型猪骨骼肌、 肝脏及胰腺组织 PKB 蛋白表达及磷酸化水平明显降低

关键词: 糖尿病, 2 型, 蛋白激酶类, 猪, 雏型, 肌, 骨骼, 肝, 胰腺, 巴马小型猪

Abstract: Abstract:Objective To study the feasibility of Bama miniature pig model establishment of type 2 diabetes mellitus (T2DM) by high-fat and high-sugar diet combined with streptozotocin (STZ), and observe the changes in protein kinase B (PKB) and phosphorylation expressions in skeletal muscle, liver, and pancreatic tissues of the miniature pig model. Methods A total of 10 healthy male Bama miniature pigs were randomly divided into two groups:control group (n=5, fed normal diet) and diabetic model group (n=5, fed high-fat and high-sugar diet combined with STZ to establish T2DM Bama miniature pig model). The fasting plasma glucose (FPG) and fasting insulin (FINS) levels were measured, and insulin resistance (HOMA-IR) was calculated in two groups. The PKB and phosphorylation expressions in skeletal muscle, liver and pancreatic tissues were measured using Western blot assay. Results (1) After 10 months of high-fat and high-sugar diet, the body weight, FPG, FINS and HOMA-IR were significantly higher in model group than those of control group (P < 0.01). (2) After STZ treatment, compared with control group, there was a further increased level of FPG and a significantly decreased level of FINS in model group (P < 0.01). (3) Compared with control group, the PKB and phosphorylation expression levels in skeletal muscle, liver and pancreas were significantly lower in model group (P＜0.05). Conclusion The high-fat and high sugar diet combined with STZ can successfully establish the T2DM miniature pig model. The PKB and phosphorylation expression levels in skeletal muscle, liver, and pancreatic tissues are decreased in model pigs.

Key words: diabetes mellitus, type 2, protein kinases, swine, miniature, muscle, skeletal, liver, pancreas, Bama miniature pig