天津医药

天津医药 ›› 2015, Vol. 43 ›› Issue (1): 38-41.doi: 10.3969/j.issn.0253-9896.2015.01.010

• 实验研究 • 上一篇    下一篇

清热合剂对上呼吸道感染常见致病菌的体外抑菌试验

马明坤 1， 闫卫利 1， 魏葆琳 2， 蒋志云 1， 李桐 1， 温学红 1△   

  1. 1 天津中医药大学第二附属医院检验科（邮编 300150）；2 肺病科
  • 收稿日期:2013-12-11 修回日期:2014-06-04 出版日期:2015-01-15 发布日期:2015-01-30
  • 通讯作者: △温学红,通讯作者 E-mail： 13662199693@163.com E-mail:13702165600@163.com
  • 基金资助:
    国家自然科学基金面上项目（81373849）

In vitro antibacterial test of Chinese medicine compound preparation for common pathogenic bacteria in upper respiratory tract infection

MA Mingkun1, YAN Weili1, WEI Baolin2, JIANG Zhiyun1, LI Tong1, WEN Xuehong1△   

  1. 1 Department of Clinical Laboratory, 2 Department of Pulmonary Medicine， the Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300150, China
  • Received:2013-12-11 Revised:2014-06-04 Published:2015-01-15 Online:2015-01-30
  • Contact: △WEN Xuehong,Corresponding Author E-mail:13662199693@163.com E-mail:13702165600@163.com

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摘要： 摘要： 目的 观察清热合剂对上呼吸道感染常见致病菌的体外抑菌活性。 方法 上呼吸道感染常见致病菌 163 株中不产超广谱 β-内酰胺酶（ESBLs）革兰阴性菌 74 株（大肠埃希菌 33 株， 肺炎克雷伯菌 24 株， 铜绿假单胞菌 17 株）；产 ESBLs 革兰阴性菌 10 株（大肠埃希菌 6 株， 肺炎克雷伯菌 4 株）；革兰阳性菌 79 株[耐甲氧西林金黄色葡萄球菌（MRSA）11 株、甲氧西林敏感金黄色葡萄球菌（MSSA）46 株， 肺炎链球菌 22 株]。 采用琼脂稀释法对清热合剂进行定量抑菌试验， 配制含有不同药物浓度的琼脂平板， 在平板上接种待测菌株菌悬液， 孵育后观察含药平板， 记录最低抑菌浓度（MIC）。 结果 清热合剂对大肠埃希菌、肺炎克雷伯菌、铜绿假单胞菌等革兰阴性菌的 MIC90 分别为 88、 176、22 g/L， 其对产 ESBLs 与不产 ESBLs 革兰阴性菌的抑菌效果一致； 不同浓度药物对铜绿假单胞菌的累积抑菌率均最高。 清热合剂对 MSSA、MRSA 和肺炎链球菌等革兰阳性菌的 MIC90 分别为 11、11、22 g/L， MRSA 的 MIC90 与 MSSA 相同， 但 MIC50 略高于 MSSA； 不同浓度药物对 MSSA 和 MRSA 的累积抑菌率均高于肺炎链球菌， 对 MSSA 与 MRSA 的累积抑菌率相近。 结论 清热合剂对上呼吸道感染常见的致病菌除肺炎克雷伯菌之外均有一定的抑菌作用， 对革兰阳性菌的抑菌效果明显优于革兰阴性菌。

关键词: 关键词： 呼吸道感染, 微生物敏感性试验, 革兰阴性菌, 革兰阳性菌, 致病菌, 琼脂稀释法, 清热合剂

Abstract: Abstract:Objective To observe the antimicrobial effect of a kind of Chinese medicine Qingre compound preparation on the common pathogenic bacteria of upper respiratory tract infection (URTI). Methods A total of 163 common pathogen⁃ ic bacteria of URTI was selected in this study, including 74 non extended-spectrum β- lactamases (ESBLs)-producing Gram-negative bacteria (33 Escherichia coli, 24 Klebsiella pneumonia and 17 Pseudomonas aeruginosa), 10 ESBLs-produc⁃ ing Gram-negative bacteria (6 Escherichia coli and 4 Klebsiella pneumoniae) and 79 Gram-positive bacteria [11 methicil⁃ lin-resistant Staphylococcus aureus (MRSA), 46 methicillin-sensitive Staphylococcus aureus and 22 Streptococcus pneu⁃ moniae]. Agar dilution method was adopted to perform the quantitative drug sensibility test. Agar plates that contained differ⁃ ent concentrations of Qingre compound preparation were prepared. The bacterial suspension was planted on the plates. Then we observed the plates after incubation, and recorded the minimum inhibitory concentration (MIC). Results The antimicro⁃ bial rates of Qingre compound preparation were 88, 176 and 22 g/L for MIC90 of Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa. The antimicrobial effects of Qingre compound preparation were coincident on the MIC90 of ES⁃ BLs-producing strains and non ESBLs-producing strains. The accumulated antibacterial rates of different concentrations of medicine to Pseudomonas aeruginosa were the highest. The MIC90 values of Qingre compound preparation were 11, 11 and 22 g/L for MSSA, MRSA and Streptococcus pneumoniae. The MIC90 of MRSA was coincident with MSSA, but MIC50 of MRSA was slightly higher than that of MSSA. The accumulated antibacterial rates of different concentrations of medicine to MSSA and MRSA were all higher than those of Streptococcus pneumonia. The accumulated antibacterial rate of MSSA was similar with that of MRSA. Conclusion The Chinese medicine Qingre compound preparation could restrain common patho⁃ genic bacteria of URTI except Klebsiella pneumoniae. The antibacterial effect of Qingre compound preparation is significant⁃ ly better in Ggram-positive bacteria than that of Gram-negative bacteria.

Key words: respiratory tract infections, microbial sensitivity tests, Gram-negative bacteria, Gram-positive bacteria, pathogenic bacteria, agar dilution method, qingre compound preparation