天津医药

天津医药 ›› 2015, Vol. 43 ›› Issue (3): 233-236.doi: 10.11958/j.issn.0253-9896.2015.03.003

• 细胞与分子生物学 • 上一篇    下一篇

新型三维复合仿生网络的构建及其组织相容性评价

崔曼 1， 张欣 1， 马丽桃 1， 熊艳杰 1， 车鹏程 1， 姚芳莲 2， 孙红 1△   

  1. 1 河北联合大学基础医学院病理教研室 （邮编 063000）； 2 天津大学化工学院
  • 收稿日期:2014-09-03 修回日期:2014-10-29 出版日期:2015-03-15 发布日期:2015-03-15
  • 通讯作者: 孙红 E-mail:501176795@qq.com
  • 作者简介:崔曼 （1987）， 女， 硕士在读， 主要从事组织工程方面研究
  • 基金资助:
    国家自然科学基金资助项目（81101448）； 河北省自然科学基金资助项目（C2011401006, H2012401017）； 河北省引进留学人员资助项目 （C201400560）

Construction of novel 3-D composite bionic network and evaluation of its histocompatibility

CUI Man1， ZHANG Xin1， MA Litao1， XiongYanjie1， CHE Pengcheng1， YAO Fanglian2， SUN Hong1△   

  1. 1 Department of Pathology, Basic Medical Sciences, Hebei United University, Tangshan 063000, China；2 School of Chemical Engineering and Technology, Tianjin University
  • Received:2014-09-03 Revised:2014-10-29 Published:2015-03-15 Online:2015-03-15
  • Contact: SUN Hong E-mail:501176795@qq.com

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摘要： 目的 构建并评价新型三维复合仿生网络的组织相容性。方法 采用仿生学方法， 将壳聚糖、 羟基磷灰石、 明胶、 果胶按一定比例制成新型三维复合仿生网络， 将小鼠胚胎成骨细胞 MC3T3-E1 与材料进行复合培养， 通过倒置相差显微镜、 石蜡切片常规染色、 扫描电镜、 F-DA 荧光染色法评价细胞相容性； 将制备好的生物支架材料植入SD 大鼠的背部皮下， 术后 2、 4、 8、 12 周评价组织相容性、 血管化能力及体内降解情况。结果 新型三维复合仿生网络呈三维多孔状， 细胞在材料上贴附生长良好， 呈多角形或梭形， 形态饱满； 皮下包埋实验发现： 早期有轻微的炎症反应， 随时间延长而消退， 后期有血管化发生， 材料降解吸收比较缓慢。结论 新型三维复合仿生网络组织相容性好， 易于血管化， 是一种很好的骨组织工程支架材料。

关键词: 新生血管化, 生理性； 组织相容性； 组织工程； 壳聚糖； 三维复合仿生网络

Abstract: Objective To construct novel 3-D composite bionic network and evaluate the histocompatibility . Methods The novel 3-D composite bionic network was prepared from chitosan, hydroxyapatite, gelatin and pectin in certain ratio by biomimtic approach, which was co-cultured with MC3T3-E1. The cell compatibility was studied by using inverted phase contrast microscope, routine paraffin section staining, scanning electron microscopy and F-DA staining. The resultant scaffold material was implanted into the dorsal subcutaneous space of SD rats. The histocompatibility, blood vessel capabilities and the degradation of the material were observed 2, 4, 8 and 12 weeks after surgery. Results The structure of novel 3-D composite bionic network was three-dimensional and porous. The cells attached on scaffolds attached and grew well with polygonal or fusiform form. It was found that inflammatory reactions were alleviated gradually in the early stage . There was an increasing angiogenesis at late stage. Materials degraded and absorbed more slowly. Conclusion The present study suggests that the novel 3-D composite bionic network has good histocompatibility with easy vascularization, and will be a candidate scaffold for bone tissue engineering.

Key words: neovascularization, physiologic, histocompatibility, tissue engineering, chitosan, 3-D composite bionic network