天津医药

天津医药 ›› 2016, Vol. 44 ›› Issue (2): 159-161.doi: 10.11958/58038

• 细胞与分子生物学 • 上一篇    下一篇

不同浓度七氟醚对人前列腺癌细胞增殖与侵袭能力的影响

王国武， 李超， 胡啸玲   

  1. 南华大学附属第一医院麻醉科
  • 收稿日期:2014-12-22 修回日期:2015-07-28 出版日期:2016-02-15 发布日期:2016-02-15
  • 基金资助:
    湖南省中医药科研计划项目 （201250）； 衡阳市科技计划项目 （2012KJ46）

The effects of different concentrations of sevoflurane on proliferation and invasion in human prostate cancer cells

WANG Guowu, LI Chao, HU Xiaoling   

  1. Department of Anesthesiology, The First Affiliated Hospital, University of South China, Hengyang 421001, China
  • Received:2014-12-22 Revised:2015-07-28 Published:2016-02-15 Online:2016-02-15

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摘要： 目的 观察不同浓度七氟醚对人前列腺癌 PC3 细胞增殖与侵袭的影响。方法 将 PC3 细胞随机分为 4 组： 对照组、 Treat1 组、 Treat2 组和 Treat3 组。对照组仅通入 O2及 CO2， Treat1～3 组在此基础上经 1.7%、 3.4%、 5.1% 七氟醚分别作用 2、 4、 6 h。MTT 检测各组细胞的增殖能力； Transwell 法检测各组细胞的侵袭能力； Western blot 检测各组细胞中缺氧诱导因子-1α（HIF-1α）蛋白表达水平。结果 与处理前相比， 处理 2、 4 和 6 h 后， 人前列腺癌 PC3 细胞的增殖和侵袭能力均明显增强 （均 P＜0.05）， 细胞中 HIF-1α的蛋白表达水平明显上调 （P＜0.05）， 且 Treat3 组＞ Treat2 组＞Treat1 组（P＜0.05）。结论 七氟醚可增强人前列腺癌 PC3 细胞的增殖与侵袭能力， 且随着时间和浓度变化细胞的增殖与侵袭能力逐渐增强， 其机制可能与上调 HIF-1α的表达有关。

关键词: 前列腺肿瘤, 麻醉, 吸入, 醚类, 细胞增殖, 肿瘤侵润, 七氟醚, 缺氧诱导因子-1α

Abstract: Objective To investigate the effects of different concentrations of sevoflurane on proliferation and invasion in human prostate cancer PC3 cell line. Methods The cells were randomly divided into four groups： control group and three sevoflurane groups (exposed to 1.7%, 3.4% and 5.1% sevollurane for 2, 4 and 6 h respectively). The ability of prolifera⁃ tion and invasion of PC3 cells were evaluated by MTT and Transwell invasion assays. Western blot analysis was performed to detect the protein expression of hypoxia inducing factor 1 alpha (HIF-1α) in PC3 cells. Results The ability of proliferation and invasion of PC3 cells was significantly increased after being exposed to sevollurane for 2, 4 and 6 h (P＜0.05), and the expression of HIF-1α protein in PC3 cells was significantly higher after being exposed to sevollurane for 2， 4 and 6 h (P＜ 0.05), showing the Treat 3 group＞ Treat 2 group＞Treat 1 group (P＜0.05). Conclusion Sevoflurane can promote prolifer⁃ ation and invasion in PC3 cells, which are gradually increased with time and concentration of sevoflurane treatment. The mechanism may involve in the up-regulation of HIF-1α expression．

Key words: prostatic neoplasms, anesthesia, inhalation, ethers, cell proliferation, neoplasm invasiveness, sevoflurane, HIF-1α