天津医药

天津医药 ›› 2016, Vol. 44 ›› Issue (6): 691-693.doi: 10.11958/20150439

• 实验研究 • 上一篇    下一篇

大鼠放射性脑损伤所致血脑屏障通透性改变与EBA 及 VEGF 表达的相关性研究

陈泊霖 1， 孙熠 2， 梁宾 3， 金学隆 1   

  1. 1天津医科大学基础医学院生理学与病理生理学系 （邮编 300070）； 2天津市第三中心医院麻醉科； 3天津市西青医院病理科
  • 收稿日期:2015-12-30 修回日期:2016-02-04 出版日期:2016-06-15 发布日期:2016-07-04
  • 基金资助:
    科技部国家科技计划项目国际科技合作专项 （2011DFA30550）

A correlation study of blood-brain barrier permeability changes and EBA, VEGF expression in rats following radiation-induced brain injury

CHEN Bolin1, SUN Yi2, LIANG Bin3, JIN Xuelong1   

  1. 1 Department of Physiology and Pathophysiology, Tianjin Medical University, Tianjin 300070, China; 2 Department of Anesthesiology, Tianjin Third Central Hospital; 3 Department of Pathology, Tianjin Xiqing Hospital
  • Received:2015-12-30 Revised:2016-02-04 Published:2016-06-15 Online:2016-07-04

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摘要： 摘要： 目的 探究脑皮质内皮屏障抗原 （EBA） 及血管内皮生长因子 （VEGF） 在放射性血脑屏障损害条件下的动态变化规律， 为临床提供参考。方法 使用随机数字表法将 48 只清洁级雄性 SD 大鼠分为对照组和放射性脑损伤后 7、 14 和 28 d 4 组， 每组 12 只。采用 X 线电子计算机断层扫描设备制备大鼠放射性脑损伤模型。大鼠按照 3 mL/kg的剂量尾静脉注射 3%伊文思蓝 （EB）， 开颅并暴露脑皮质血管， 放于微循环显微镜下观察 EB 渗出情况， 并借助微循环显微成像系统评估血脑屏障通透性； 使用免疫组化染色的方法来检测各组大鼠脑皮质 EBA 和 VEGF 的表达。结果 与对照组相比， 损伤组大鼠脑皮质微血管 EB 外渗量和 VEGF 的表达水平于伤后 7、 14、 28 d 均有不同程度升高（均 P＜0.05）， 从伤后 7 d 至 28 d 逐渐降低， 损伤各亚组之间差异均有统计学意义（均 P＜0.05）， 二者呈正相关（r=0.898， P < 0.001）； EBA 表达水平于伤后各个时间点均下降 （均 P＜0.05）， 从伤后 7 d 至 28 d 逐渐升高， 损伤各亚组之间差异均有统计学意义 （均 P＜0.05）， 与 EB 外渗量呈负相关 （r=-0.866， P < 0.001）。结论 大鼠放射性脑损伤后血脑屏障通透性增加与 EBA 表达减少、 VEGF 表达增加具有重要关联性。

关键词: 辐射损伤, 实验性, 脑损伤, 血脑屏障, 血管内皮生长因子类, 大鼠, Sprague-Dawley, 放射性脑损伤, 内皮屏障抗原

Abstract: Abstract: Objective To explore the dynamic changes of endothelial barrier antigen (EBA) and vascular endothelial growth factor (VEGF) expressions in cerebral cortex under the condition of blood-brain barrier damage in rats following radiation-induced brain injury, which provided clinical references. Methods Forty-eight clean grade male SD rats were divided into the control group and 7 d, 14 d, 28 d after brain irradiation group (n=12 for each group) by using stochastic indicator method. The radiation-induced brain injury model was established by using electronic computer X-ray tomography technique. The 3% Evans blue (EB) was injected into rats according to the dose of 3 mL/kg via the tail vein, then the blood vessels of cerebral cortex were exposed after having a craniotomy. EB extravasation was detected by microcirculation microscope. The permeability of blood-brain barrier was evaluated by using microscope vascular camera device. The expressions of EBA and VEGF in the cerebral cortex were measured by immunohistochemistry staining in each group. Results Both of EB extravasation and VEGF expression in rat cerebral cortex were significantly increased in injury group at day 7, 14 and 28 after brain irradiation compared with those of control group (P＜0.05), and which were gradually decreased from day 7 to day 28 after brain irradiation. There were significant differences in EB extravasation and VEGF expression between the injury subgroups (P＜0.05). There was a positive correlation between EB extravasation and VEGF expression (r=0.898, P < 0.001).The expression levels of EBA were decreased at different time points in injury groups compared with those of control group(P＜0.05), and gradually increased from day 7 to 28 after injury. There were significant differences in expression levels ofEBA between injury subgroups (P＜0.05). The expression of EBA was negatively correlated with EB extravasation (r=-0.866,P < 0.001). Conclusion The increases of blood-brain barrier permeability have important relation to the decreases of EBA expression and the increases of VEGF expression after radiation-induced brain injury.

Key words: radiation injuries, experimental, brain injuries, blood-brain barrier, vascular endothelial growth factors, rats, Sprague-Dawley, radiation-induced brain injury, endothelial barrier antigen rats, Sprague-Dawley, radiation-induced brain injury, endothelial barrier antigenendothelial growth factor, rats