天津医药

天津医药 ›› 2016, Vol. 44 ›› Issue (7): 843-845.doi: 10.11958/20160135

• 细胞与分子生物学 • 上一篇    下一篇

人诱导多能干细胞重编程过程长链非编码RNA的差异表达研究

俞茜,刘雅丽,范勇   

  1. 广州， 广州医科大学附属第三医院， 广东省产科重大疾病重点实验室（邮编 510150）
  • 收稿日期:2016-03-09 修回日期:2016-04-17 出版日期:2016-07-15 发布日期:2016-07-15
  • 通讯作者: 范勇 E-mail:185876976@qq.com
  • 作者简介:俞茜（1990）， 女， 硕士在读， 主要从事干细胞与生殖方面的研究
  • 基金资助:
    国家自然科学基金资助项目（81370766）

Studies on the long non-coding RNA during the reprogramming of human pluripotent stem cells

YU Qian, LIU Yali, FAN Yong   

  1. Key Laboratory for Major Obstetric Diseases of Guangdong Province, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou 510150, China
  • Received:2016-03-09 Revised:2016-04-17 Published:2016-07-15 Online:2016-07-15
  • Contact: FAN Yong E-mail:185876976@qq.com

PDF (PC)

3703

摘要： 目的 探讨人诱导多能细胞重编程过程中长链非编码RNA的表达改变及其作用。方法 利用Agilent Human lncRNA (4*180K)芯片检测人体细胞、诱导多能干细胞以及胚胎干细胞中的lncRNA表达情况。通过数据分析比较人体细胞诱导为多能干细胞后lncRNA的差异表达,筛选出在重编程过程中可能发挥作用的lncRNA。结果 人诱导多能干细胞lncRNA表达谱类似于胚胎干细胞而与体细胞不同。通过聚类分析发现干细胞与体细胞之间有3 156个差异表达lncRNA。通过生物学分析发现人体细胞诱导为多能干细胞重编程过程中有222个差异表达的lncRNA。结论 lncRNA在人多能干细胞重编程过程中可能发挥着重要的作用。

关键词: 长链非编码RNA, 多能干细胞, 核重编程, 芯片分析技术

Abstract: Abstract: Objective To investigate the changes and roles of the Long non-coding RNA during the reprogramming of human induced pluripotent stem cells. Methods Using Agilent Human lncRNA (4 * 180K) chip to check the of lncRNA in somatic cells, induced pluripotent stem cells and embryonic stem cells. Compared with differentially expressed lncRNA in somatic cells and induced pluripotent stem cells, lncRNA may play an important role during the reprogramming of human pluripotent stem cells. Results The lncRNA profiles in induced pluripotent stem cells are similar to embryonic stem cells but are different from the somatic cells． 3156 differentially expressed lncRNAs are found between stem cells and somatic cells by cluster analysis. 222 differentially expressed lncRNAs are found between during the reprogramming process of human pluripotent stem cells by biological analysis. Conclusion lncRNA may play an important role in human pluripotent stem cell reprogramming process.

Key words: Long non-coding RNA, human pluripotent stem cell, reprogramming, Arrary