天津医药

天津医药 ›› 2017, Vol. 45 ›› Issue (8): 830-832.doi: 10.11958/20170348

• 专题 颅脑神经创伤 • 上一篇    下一篇

神经干细胞移植对脑外伤大鼠神经功能的影响

马玉东 1△， 明宗毅 2   

  1. 1 沈阳医学院附属中心医院重症医学科 （邮编 110024）， 2 神经外科
  • 收稿日期:2017-03-20 修回日期:2017-07-12 出版日期:2017-08-15 发布日期:2017-08-15
  • 通讯作者: △通讯作者 E-mail: 1433481507@qq.com E-mail:1433481507@qq.com
  • 作者简介:马玉东 （1975）， 男， 硕士， 副主任医师， 主要从事重症医学研究

Effects of neural stem cell transplantation on nerve function of rats with traumatic brain injury

MA Yu-dong1△, MING Zong-yi2   

  1. 1 Intensive Care Unit Central, 2 Department of Neurosurgery, the Affiliated Hospital of Shenyang Medical College, Shenyang 110024, China
  • Received:2017-03-20 Revised:2017-07-12 Published:2017-08-15 Online:2017-08-15
  • Contact: △Corresponding Author E-mail: 1433481507@qq.com E-mail:1433481507@qq.com

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摘要： 目的 探讨神经干细胞移植对脑外伤大鼠神经功能及脑组织 Caspase-3 和 Caspase-9 表达的影响。方 法 108 只 SD 成年大鼠随机分为假手术 （Sham） 组、 脑外伤 （TBI） 组和神经干细胞移植 （NSC） 组， 每组 36 只。TBI 和 NSC 组采用改良的 Feeney 氏法制作大鼠脑外伤动物模型， NSC 组大鼠在脑外伤模型制作成功后 1 周在皮质处局部 注射移植神经干细胞， TBI 和 NSC 组大鼠分别在细胞移植后的第 7 天、 14 天和 21 天进行神经功能缺损评分 （NSS）； sham 组仅切开头皮暴露颅骨， 不实施打击。免疫组织化学方法和 Western blot 方法检测各组大鼠脑组织 Caspase-3 和 Caspase-9 蛋白的表达。结果 各时点 NSC 组大鼠较 TBI 组 NSS 评分均降低（P＜0.01）。免疫组织化学方法和 Western blot 均显示， 与 Sham 组相比， TBI 组大鼠脑组织的 Caspase-3 和 Caspase-9 阳性表达 OD 值均升高（P＜ 0.05）； 而与相同时间点的 TBI 组大鼠相比， NSC 组大鼠脑组织 Caspase-3 和 Caspase-9 的阳性表达 OD 值均降低， 但 仍高于 Sham 组（P＜0.05）。结论 神经干细胞移植后可能通过下调 Caspase-3 和 Caspase-9 的表达促进脑外伤大 鼠神经功能的恢复。

关键词: 脑损伤, 干细胞移植, 神经元, 半胱氨酸天冬氨酸蛋白酶 3, 半胱氨酸天冬氨酸蛋白酶 9, 疾病模型, 动物

Abstract: Objective To study the effect of neural stem cell transplantation on neurological behavior and expressions of Caspase-3 and Caspase-9 in rats with traumatic brain injury (TBI). Methods A total of 108 SD rats were randomly divided into the sham group (n=36), traumatic brain injury group (TBI, n=36) and neural stem cell transplantation group (NSC, n=36). TBI model of rats was established by using improved Feeney method. Rats of NSC group were injected neural stem cells in cortex after traumatic brain injury was established for 1 week, and then nurological severity scores (NSS) of rats were evaluated at 7 days, 14 days and 21 days after cell transplantation in TBI and NSC groups, respectively. Rats of sham group were only cut the scalp and exposed the skull without striking. The expressions of Caspase-3 and Caspase-9 in brain tissues were detected by immunohistochemistry and Western blot method. Results Compared with NSC group at the same time points, NSS was decreased significantly in TBI group (P<0.01). Compared with the sham group, the optical density (OD) values of Caspase-3 and Caspase-9 positive expressions were increased significantly in TBI group (P<0.05). Compared with TBI group at the same time points, the OD values of Caspase- 3 and Caspase- 9 positive expressions were decreased significantly in NSC group (P<0.05). Conclusion The neural stem cell transplantation can promote the recovery of brain injury by down-regulating the expressions of Caspase-3 and Caspase-9.

Key words: brain injuries, stem cell transplantation, neurons；caspase 3, caspase 9, disease models, animal