天津医药

天津医药 ›› 2019, Vol. 47 ›› Issue (10): 1009-1014.doi: 10.11958/20192305

• 细胞与分子生物学 •    下一篇

骨碎补总黄酮通过SDF1/CXCR4信号途径促进小鼠 骨髓基质细胞ST-2迁移

吴紫璇 1，郝征 2，郭亚萍 1，张翟轶 1，陈璐瑶 1，彭雁飞 1△，郑纺 1△   

  1. 1天津中医药大学中西医结合学院（邮编301617），2中医学院
  • 收稿日期:2019-07-28 修回日期:2019-09-19 出版日期:2019-10-15 发布日期:2019-11-11
  • 通讯作者: 郑纺 E-mail:cfq_zf@tjutcm.edu.cn
  • 基金资助:
    国家自然科学基金青年项目;天津市自然科学基金面上项目;天津市自然科学基金面上项目

Assemble flavone of rhizome drynaria promoted the migration of mouse ST-2 cells through SDF-1/CXCR4 signaling pathway

WU Zi-xuan1, HAO Zheng2, GUO Ya-ping1, ZHANG Zhai-yi1, CHEN Lu-yao1, PENG Yan-fei1△, ZHENG Fang1△   

  1.  1 School of Integrative Medicine, 2 School of Traditional Chinese Medicine,Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
  • Received:2019-07-28 Revised:2019-09-19 Published:2019-10-15 Online:2019-11-11
  • Supported by:
     

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摘要： 摘要：目的 探讨骨碎补总黄酮（AFRD）对小鼠骨髓基质细胞系ST-2迁移能力及SDF1/CXCR4信号途径的调节 作用。方法 以乙醇超声提取法从强骨胶囊中获取AFRD，采用CCK-8法确定不同浓度AFRD（5、20、50、100 mg/L） 对ST-2细胞增殖的影响；Transwell实验检测AFRD对ST-2迁移能力的影响；使用荧光定量PCR和Western blot检测 AFRD对SDF1和CXCR4基因表达的影响；CXCR4抑制剂AMD3100干预ST-2细胞后再次观察AFRD对ST-2细胞迁 移能力及SDF1和CXCR4基因表达的变化。结果 AFRD促进ST-2细胞的迁移，上调细胞中SDF1和CXCR4基因的表 达，并呈现浓度依赖性。CXCR4抑制剂AMD3100能够在一定程度上逆转AFRD对ST-2细胞迁移的促进作用及SDF1 和CXCR4基因表达的上调效应。结论 AFRD能够促进ST-2的迁移，该作用是通过SDF1/CXCR4信号途径介导的。

关键词: 骨碎补, 细胞运动, 趋化因子CXCL12, 骨髓基质细胞, SDF1, CXCR4

Abstract: Abstract: Objective To investigate the effect of assemble flavone of rhizome drynaria (AFRD) on the migration and regulation of SDF1 / CXCR4 signaling pathway in murine bone marrow stromal ST-2 cell line. Methods AFRD was obtained from qianggu capsule by ethanol ultrasonic extraction, CCK-8 method was used to determine the effects of AFRD (5, 20, 50 and 100 mg / L) on the proliferation of ST-2 cells. Transwell was used to detect the effect of AFRD on ST-2 migration ability. Fluorescence quantitative PCR and Western blot assay were used to detect the effect of AFRD on SDF1 and CXCR4 gene expressions. After intervention with CXCR4 inhibitor AMD3100, the migration ability and gene expression of SDF1 and CXCR4 regulated by AFRD in ST-2 cells were observed. Results AFRD promoted the migration of ST-2 cells and up-regulated the expressions of SDF1 and CXCR4 genes in ST-2 cells in a concentration-dependent manner. AMD3100, a CXCR4 inhibitor, can reverse the effect of AFRD on ST-2 cell migration and the upregulation of SDF1 and CXCR4 gene expression. Conclusion AFRD can promote ST-2 migration, which is mediated by SDF1/CXCR4 signaling pathway.

Key words: Drynaria Fortunei, cell movement, chemokine CXCL12, bone marrow stromal cells, SDF1, CXCR4

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