天津医药

天津医药 ›› 2020, Vol. 48 ›› Issue (6): 522-526.

• 实验研究 • 上一篇    下一篇

富含血小板血浆修复小鼠皮肤组织缺损创面的实验研究

刘思婧 1，张潇 2，李益 1，罗茂 1，吴剑波 1，李蓉 1△
  

  1. 1西南医科大学药物与功能性食品研究中心（邮编646000）；2西南医科大学基础医学院
  • 收稿日期:2019-10-17 修回日期:2020-03-27 出版日期:2020-06-15 发布日期:2020-06-15
  • 通讯作者: 李蓉 E-mail:42951187@qq.com
  • 基金资助:
    血小板介导肿瘤血管形成和稳定性的机制研究;脂肪移植对II型糖尿病小鼠代谢和下肢缺血血流灌注的影响

The experimental study of platelet-rich plasma in repairing skin wound defects in mice #br#

LIU Si-jing1, ZHANG Xiao2, LI Yi1, LUO Mao1, WU Jian-bo1, LI Rong1△ #br#   

  1. 1 Drug and Functional Food Research Center, Southwest Medical University, Luzhou 646000, China;
    2 School of Basic Medicine, Southwest Medical University
  • Received:2019-10-17 Revised:2020-03-27 Published:2020-06-15 Online:2020-06-15

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摘要： 摘要：目的 研究富含血小板血浆（PRP）的不同处理方式对小鼠皮肤创伤愈合的影响。方法 清洁级健康雄性 C57BL/6J小鼠27只，其中12只用于制备PRP，另外15只小鼠根据随机数字表法将其分为对照组、PRP注射组以及 PRP凝胶组，每组5只。3组小鼠均建立皮肤创伤模型。PRP注射组将小鼠皮肤伤口等分6点皮下注射PRP，每点注 入0.1 mL；对照组按照相同方法注射等量生理盐水；PRP凝胶组将PRP激活制备成凝胶后覆盖在每只小鼠皮肤伤口 上。3组小鼠均仅在术后当天给药1次，并分别于当天，第3、7、11、14天拍照时计算伤口面积。第14天处死3组小 鼠，收集创口处皮肤组织，用免疫荧光染色评价胶原蛋白表达；实时荧光定量PCR检测Ⅰ型胶原α1链（COL1A1）、Ⅲ 型胶原α1链（COL3A1）、转化生长因子-β1（TGF-β1）、血小板源性生长因子（PDGF）、血管内皮生长因子（VEGF）的 mRNA表达。结果 与对照组和PRP注射组相比，在术后不同时间点，PRP凝胶组的皮肤伤口明显愈合且伤口面积 减小（P＜0.05）。术后第11、14天，与对照组相比，PRP注射组伤口面积减小（P＜0.05）。术后第14天，与对照组和 PRP注射组相比，PRP凝胶组胶原蛋白表达明显增加，VEGF、COL1A1、COL3A1、TGF-β1、PDGF的mRNA水平升高 （P＜0.05）。结论 将PRP激活制备成凝胶可有效修复小鼠皮肤组织缺损创面

关键词: 伤口愈合, 皮肤, 富血小板血浆, 凝胶类, 胶原

Abstract: Abstract: Objective To study the effects of different treatment methods of platelet-rich plasma (PRP) on skin wound healing in mice. Methods A total of 27 C57BL/6J male mice were used in this study, 12 of them were used to prepare PRP, and the other 15 mice were randomly divided into control group, PRP injection group and PRP gel group. There were 5 mice in each group. Models of skin injury were established in all three groups of mice. For the PRP injection group, the skin wound was equally divided into 6 points and subcutaneously injected PRP with 0.1 mL at each point. The control group was injected the same amount of saline with the same method. For the PRP gel group, PRP was activated to prepare gel to cover the skin wounds of mice. All three groups were given medicine only once on the day after surgery. The wound areas were photoed and calculated on the 0, 3, 7, 11, and 14 day respectively. On the 14th day, mice of the three groups were sacrificed and the skin tissues around the wound were collected. The collagen expression was evaluated by immunofluorescence staining. The mRNA expressions of collagen type Ⅰ alpha 1 chain (COL1A1), collagen type Ⅲ alpha 1 chain (COL3A1), transforming growth factor-beta 1 (TGF-β1), platelet derived growth factor (PDGF) and vascular endothelial growth factor (VEGF) in the wound site were detected by qRT-PCR. Results Compared with the control group and the PRP injection group, the skin wounds healed significantly and the wound area decreased in the PRP gel group at different time points after surgery (P＜0.05). At 11 and 14 days after surgery, the wound area decreased in the PRP injection group compared with that of the control group (P＜0.05). At 14 days after operation, collagen expressions were significantly increased in the PRP gel group compared with those of control group and the PRP injection group, and mRNA levels of VEGF, COL1A1, COL3A1, TGF-β1 and PDGF increased (P＜0.05). Conclusion The preparation of gel from the PRP activation can effectively repair the skin defects of mice.

Key words: wound healing, skin, platelet-rich plasma, gels, collagen