天津医药

天津医药 ›› 2020, Vol. 48 ›› Issue (7): 676-681.doi: 10.11958/20193431

• 综述 • 上一篇    下一篇

中性粒细胞胞外诱捕网检测技术的研究进展

陈玉年1 ，顾兵1 ，李华南2△   

  1. 1 江西科技师范大学药学院（邮编 330013）；2 江西中医药大学附属医院
  • 收稿日期:2019-11-12 修回日期:2020-03-13 出版日期:2020-07-15 发布日期:2020-07-16
  • 作者简介:1 江西科技师范大学药学院（邮编 330013）；2 江西中医药大学附属医院

Overview of detection techniques for neutrophil extracellular traps

CHEN Yu-nian1 , GU Bing1 , LI Hua-nan2△   

  1. 1 College of Pharmacy, Jiangxi Science & Technology Normal University, Nanchang 330013, China; 2 the Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine
  • Received:2019-11-12 Revised:2020-03-13 Published:2020-07-15 Online:2020-07-16

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摘要： 摘要：中性粒细胞胞外诱捕网（NETs）是近些年新发现的，与自身免疫性疾病、血栓和炎症反应形成有关的网状 结构，由中性粒细胞在触发因子诱导下释放产生。不同的刺激物会诱导出不同形式，其数量可被多种检测技术量 化。根据检测对象的不同，其分析方法有形态学观察，检测游离DNA和中性粒细胞弹性蛋白酶、髓过氧化物酶等蛋 白质组分。本文通过概述这些检测技术及各自优缺点，为进一步拓展NETs在相关疾病的早期诊断、预后判断等方 面的应用提供有益参考。

关键词: 中性粒细胞胞外诱捕网, 形态学和显微观察, 游离DNA, 中性粒细胞弹性蛋白酶, 髓过氧化物酶

Abstract: Abstract：Neutrophil extracellular traps (NETs) are a newly recognized structure in recent years, which is involved in the pathogenesis of autoimmune diseases, thrombosis and inflammatory reaction. NETs can be induced to different forms of reticular structure by a variety of trigger factors and can be quantified by various techniques. According to the different subjects, the analysis methods include morphological observation, detection of cell free DNA and neutrophil elastasemyeloperoxidase, citrullinated histone and other protein components. In this paper, the advantages and disadvantages of these detection techniques are summarized, which will provide useful reference for the further development of the application of NETs in the early diagnosis and prognosis judgment of related diseases.

Key words: neutrophil extracellular traps, morphological and microscopic findings, cell-free DNA, neutrophil elastase, myeloperoxidase