天津医药

天津医药 ›› 2022, Vol. 50 ›› Issue (2): 113-119.doi: 10.11958/20210892

• 细胞与分子生物学 •    下一篇

miR-1307-3p通过靶向ISM1促进乳腺癌细胞的增殖和迁移

陆旭阳,刘奕,李晓辉,黄欣,黄翠霞,马书祥,窦伟瑜,谢彬彬,曾麒燕   

  1. 1广西医科大学基础医学院生物化学与分子生物学教研室（邮编530021）；2广西高校生物分子医学研究重点实验室
  • 收稿日期:2021-04-14 修回日期:2021-08-09 出版日期:2022-02-15 发布日期:2022-02-15
  • 通讯作者: 曾麒燕 E-mail:2257994291@qq.com
  • 基金资助:
    国家自然科学基金资助项目（82060387）；广西自然科学基金资助项目（2018GXNSFAA281240）

MiR-1307-3p promotes the proliferation and migration of breast cancer cells by targeting ISM1 #br#

LU Xuyang, LIU Yi, LI Xiaohui, HUANG Xin, HUANG Cuixia, MA Shuxiang, DOU Weiyu, ZENG Qiyan #br#   

  1. 1 Department of Biochemistry and Molecular Biology, Guangxi Medical University, Nanning 530021, China;
    2 Guangxi Colleges and Universities, Key Laboratory of Biological Molecular Medicine Research
  • Received:2021-04-14 Revised:2021-08-09 Published:2022-02-15 Online:2022-02-15

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摘要：

目的 探讨miR-1307-3p通过靶向ISM1促进乳腺癌细胞增殖和迁移的分子机制。方法 （1）利用TCGA
数据库分析miR-1307-3p在乳腺癌患者中的表达水平及其与临床指标的关系。（2）利用qPCR、CCK-8实验、细胞划
痕实验和流式细胞术检测过表达或沉默miR-1307-3p后乳腺癌MCF-7细胞miR-1307-3p表达、细胞增殖、迁移和凋
亡水平变化。（3）生物信息学分析软件预测 miR-1307-3p 的靶基因，同时采用双荧光素酶实验进行验证。结果
miR-1307-3p在乳腺癌细胞及乳腺癌组织中均显著上调。miR-1307-3p过表达可促进MCF-7细胞增殖和迁移；而
miR-1307-3p inhibitor则抑制细胞迁移，并诱导凋亡。ISM1可能是miR-1307-3p的靶基因。乳腺癌组织中ISM1表
达水平明显低于正常乳腺组织，且与临床病理分期有关。 结论miR-1307-3p可促进乳腺癌细胞增殖和迁移，可能
通过调控ISM1基因而影响乳腺癌的发生发展。

关键词: 乳腺肿瘤, 微RNAs, 细胞增殖, 细胞运动, miR-1307-3p, Isthmin 1

Abstract: Objective To explore the possible molecular mechanism of miR-1307-3p in promoting proliferation and migration by targeting ISM1 in breast cancer cells. Methods (1) Cancer Genome Atlas (TCGA) database was used to analyze the expression level of miR-1307-3p and its relationship with clinical indicators in breast cancer patients. (2) qPCR, CCK-8 assay, Scratch test and flow cytometry were used to detect the effects of miR-1307-3p over-expression or silence on the expression of miR-1307-3p, cell proliferation, migration and apoptosis in breast cancer MCF-7 cells, respectively. (3) The target gene of mir-1307-3p was predicted by bioinformatics analysis, and verified by double luciferase reporter gene experiment. Results The relative expression levels of miR-1307-3p were significantly up-regulated both in breast cancer cell lines and breast cancer tissues. Overexpression of miR-1307-3p significantly promoted cell proliferation and migration in MCF-7 cells; on the contrary, miR-1307-3p inhibitor significantly inhibited cell migration and induced cell apoptosis. ISM1 may be the target genes of miR-1307-3p. The expression level of ISM1 was significantly lower in breast cancer tissues than that in normal breast tissues, and was significantly correlated with clinical stages. Conclusion MiR- 1307-3p can promote the proliferation and migration of breast cancer cells, which may affect the occurrence and development of breast cancer by targeting ISM1 gene.

Key words: breast neoplasms, microRNAs, cell proliferation, cell movement, miR-1307-3p, Isthmin 1