天津医药

天津医药 ›› 2022, Vol. 50 ›› Issue (5): 449-455.doi: 10.11958/20212087

• 细胞与分子生物学 •    下一篇

分泌磷酸蛋白1对肺腺癌A549细胞生物学特性的影响

栾艳超1，梁超1△，韩青松1，刘佳坤1，杨立伟2，李志峰2   

  1. 1河北省胸科医院胸外科（邮编050001）；2河北医科大学第四医院胸外科
  • 收稿日期:2021-09-09 修回日期:2022-01-28 出版日期:2022-05-15 发布日期:2022-07-04
  • 基金资助:
    河北省卫计委医学科学研究重点课题计划资助项目

Effects of SPP1 on biological characteristics of lung adenocarcinoma A549 cells

LUAN Yanchao1, LIANG Chao1△, HAN Qingsong1, LIU Jiakun1, YANG Liwei2, LI Zhifeng2   

  1. 1 Department of Thoracic Surgery, Hebei Chest Hospital, Shijiazhuang 050001, China;2 Department of Thoracic Surgery, the Fourth Hospital of Hebei Medical University
  • Received:2021-09-09 Revised:2022-01-28 Published:2022-05-15 Online:2022-07-04

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摘要： 目的　探讨分泌磷酸蛋白1（SPP1）在肺腺癌（LUAD）中的表达情况及对LUAD细胞生物学功能的影响。方法　通过UALCAN、GEPIA数据库分析SPP1 mRNA在LUAD中的表达情况，GEPIA、K-M数据库分析SPP1 mRNA表达与预后的关系；通过实时荧光定量PCR（qPCR）和Western blot检测SPP1在LUAD组织及细胞系中表达情况。构建SPP1干扰序列并将其转染入A549细胞中，设为Blank组（不转染）、NC组（转染NC序列）、siSPP1组（转染siSPP1序列）。Western blot检测细胞中SPP1沉默效果；CCK-8、EdU、细胞克隆形成实验检测siSPP1对LUAD细胞增殖能力的影响；TUNEL实验检测siSPP1对LUAD细胞凋亡的影响；Transwell和划痕实验检测siSPP1对LUAD细胞侵袭和迁移能力的影响。结果　生物信息数据库分析显示，SPP1 mRNA在LUAD中高表达（P＜0.05），且SPP1 mRNA高表达者累积生存率较低（P＜0.05）；LUAD组织及A549细胞中SPP1均高表达（P＜0.05）；沉默SPP1表达能够抑制A549细胞的增殖、侵袭和迁移能力，诱导细胞凋亡（P＜0.05）。结论　SPP1可作为肺腺癌诊治的潜在靶点，其可能通过调控细胞增殖、迁移及侵袭等特性影响肺腺癌的发生、发展。

关键词: 肺肿瘤, 腺癌, 细胞增殖, 细胞凋亡, 细胞运动, 分泌磷酸蛋白1

Abstract: Objective　To investigate the expression of secreted phosphoprotein 1 (SPP1) in lung adenocarcinoma (LUAD) and its effect on the biological function of LUAD cells. Methods　The expression of SPP1 mRNA in LUAD was analyzed by UALCAN and GEPIA databases. The relationship between SPP1 mRNA expression and prognosis was analyzed by GEPIA and K-M database. The expression levels of SPP1 in LUAD tissues and cell lines was detected by real-time fluorescence quantitative PCR (qPCR) and Western blot assay. The interfering sequence targeting SPP1 was constructed and transfected into A549 cells, and cells were divided into the blank group (no transfection), the NC group (transfection with SPP1 negative control sequence) and the siSPP1 group (transfection with siRNA sequence targeting SPP1). Western blot assay was used to detect the silencing effect of SPP1 in cells. The effects of siSPP1 on the proliferation of LUAD cells were detected by CCK-8, EDU and plate clonal formation experiments. TUNEL apoptosis assay was used to detect the effect of siSPP1 on apoptosis of LUAD cells. The effects of siSPP1 on the invasion and migration of LUAD cells were detected by Transwell and scratch assay. Results　Bioinformation database analysis showed that SPP1 mRNA was highly expressed in LUAD (P＜0.05). The high expression of SPP1 mRNA was negatively correlated with the overall survival (OS) of patients with LUAD (P＜0.05). SPP1 was highly expressed in LUAD and A549 cells (P＜0.05). Silencing SPP1 could inhibit the proliferation, invasion and migration of A549 cells and induce apoptosis (P＜0.05). Conclusion　SPP1 can be a potential target in the diagnosis and treatment of lung adenocarcinoma, which may affect the occurrence and development of lung adenocarcinoma by regulating cell proliferation, migration and invasion.

Key words: lung neoplasms, adenocarcinoma, cell proliferation, apoptosis, cell movement, secreted phosphoprotein l

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