天津医药

天津医药 ›› 2017, Vol. 45 ›› Issue (8): 856-859.doi: 10.11958/20170594

• 实验研究 • 上一篇    下一篇

发酵虫草菌粉对糖尿病大鼠心肌 NLRP3 表达的影响

吕元军 1， 王卓 2， 崔壮 3， 李金娥 1， 谭效锋 1， 李长平 3△   

  1. 1 天津市天津医院普通内科 （邮编 300211）； 2 天津市和平区小白楼街社区卫生服务中心； 3 天津医科大学公共卫生学院
  • 收稿日期:2017-05-24 修回日期:2017-06-26 出版日期:2017-08-15 发布日期:2017-08-15
  • 通讯作者: △通讯作者 E-mail: changpingli@163.com E-mail:lyj22337@163.com
  • 作者简介:吕元军 （1975）， 男， 博士， 副主任医师， 主要从事糖尿病慢性并发症的发病机制研究
  • 基金资助:
    国家自然科学基金青年基金项目 （81200611）； 天津市卫生局科技基金项目 （2011KY22， 2010KZ55）

Effects of fermented cordyceps powder on the expression of NLRP3 in myocardium of diabetic rats

LYU Yuan-jun1, WANG Zhuo2, CUI Zhuang3, LI Jin-e1, TAN Xiao-feng1, LI Chang-ping 3△   

  1. 1 Division of General Internal Medicine, Tianjin Hospital, Tianjin 300211, China; 2 Xiaobailou Community Health Service Centre of Heping District, Tianjin; 3 School of Public Health, Tianjin Medical University
  • Received:2017-05-24 Revised:2017-06-26 Published:2017-08-15 Online:2017-08-15
  • Contact: △Corresponding Author E-mail: changpingli@163.com E-mail:lyj22337@163.com

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摘要： 目的 探讨发酵虫草菌粉对糖尿病心肌病的治疗作用及其对心肌核苷酸结合寡聚化结构域样受体 3 （NLRP3）、 半胱氨酸天冬氨酸蛋白酶 1（Caspase-1）、 白细胞介素-1β（IL-1β）表达的影响。方法 雄性 SD 大鼠 45 只， 随机分为正常对照组 （CON 组）、 糖尿病组 （DM 组） 和发酵虫草菌粉治疗组 （CS 组， 1 g·kg-1·d-1）， 每组 15 只。DM 组和 CS 组大鼠通过尾静脉注射链脲佐菌素（45 mg/kg）诱导糖尿病模型。CS 组每日给予 1 次发酵虫草菌粉溶液灌 胃， CON 组和 DM 组给予相同体积生理盐水灌胃。灌胃 18 周后取材。HE 染色观察心肌组织形态学改变； 电镜观察 心肌超微结构改变； Western blotting 法检测心肌 NLRP3 的表达； 免疫组化法检测各组大鼠心肌 NLRP3、 Caspase-1、 IL-1β 的表达水平。结果 （1） 电镜下， DM 组大鼠出现局部心肌纤维变性坏死、 部分线粒体损伤； CS 组大鼠心肌病 变较 DM 组明显减轻。（2） Western blotting 结果显示， DM 组和 CS 组大鼠心肌 NLRP3 表达均较 CON 组显著上升， CS 组大鼠心肌 NLRP3 表达较 DM 组降低。（3）免疫组化结果显示， DM 组和 CS 组大鼠心肌 NLRP3、 Caspase-1、 IL-1β 表达均较 CON 组显著上升， CS 组均较 DM 组降低。结论 发酵虫草菌粉能减轻糖尿病大鼠心肌病变， 该作用可能 与其抑制心肌 NLRP3、 Caspase-1、 IL-1β 的表达有关。

关键词: 糖尿病并发症, 半胱氨酸天冬氨酸蛋白酶 1, 白细胞介素 1β, 糖尿病心肌病, 发酵虫草菌粉, 核苷酸结合 寡聚化结构域样受体 3

Abstract: Objective To investigate the therapy effect of fermented cordyceps powder on diabetic cardiomyopathy, and the effect on expressions of NLRP3, Caspase-1 and interleukin (IL)-1β in myocardium of diabetic rats. Methods A total of 45 male SD rats were randomly divided into normal control group (group CON), diabetes group (group DM) and fermented cordyceps powder administration group (group CS, 1 g·kg- 1·d- 1). Diabetes rat model was induced by tail vein injection with streptozotocin (45 mg/kg) in group DM and group CS. The rats in group CS were given fermented cordyceps powder solution by gavage at a single dose of 1 g·kg-1·d-1 for 18 weeks. Rats of group CON and group DM were given 0.9% NaCl in the same way. All rats were sacrificed after 18 weeks. The pathological changes in myocardium were observed by HE staining and electron microscope. The expression of NLRP3 in myocardium was detected by Western blotting. The expression levels of NLRP3, Caspase-1 and IL-1β in myocardium were detected by immunohistochemistry. Results (1) Myocardial necrosis and fibrosis and mitochondrial damages were observed under electron microscope in group DM. The above-mentioned pathological changes were improved in the group CS. (2) The result of Western blotting showed that the expression of NLRP3 in myocardium was significantly increased in group DM and group CS than that in group CON, and the expression of NLRP3 was significantly lower in group CS than that in group DM. (3) The result of immunohistochemistry showed that, compared with the group CON, the expressions of NLRP3, Caspase- 1 and IL- 1β in myocardium were significantly increased in group DM and group CS, and which were significantly lower in group CS than those in group DM. Conclusion Fermented cordyceps powder can improve diabetic cardiomyopathy through decreasing the expressions of NLRP3, Caspase-1 and IL-1β in myocardium.

Key words: diabetes complications, caspase- 1, interleukin- 1beta, diabetic cardiomyopathy, fermented cordyceps powder, NLRP3