天津医药

天津医药 ›› 2016, Vol. 44 ›› Issue (6): 724-728.doi: 10.11958/20150224

• 临床研究 • 上一篇    下一篇

hrHPV 在宫颈癌中的感染状况及对 Caspase-1 和 IL-1β 表达的影响及意义

罗远材 1， 王宝晨 2， 郭路 1， 李静 1   

  1. 1天津市第一中心医院妇产科(邮编300192)； 2天津医科大学总医院妇产科
  • 收稿日期:2015-10-14 修回日期:2016-01-10 出版日期:2016-06-15 发布日期:2016-07-04

The infection status of hrHPV and its effect and significance on expression of Caspase-1 and IL-1β in human cervical carcinoma

LUO Yuancai1, WANG Baochen2， GUO Lu1, LI Jing1   

  1. 1 Department of Obstetrics and Gynecology, Tianjin First Central Hospital， Tianjin 300192， China； 2 Department of Obstetrics and Gynecology, Tianjin Medical University General Hospital
  • Received:2015-10-14 Revised:2016-01-10 Published:2016-06-15 Online:2016-07-04

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摘要： 摘要： 目的 探讨宫颈癌中高危人乳头瘤病毒（hrHPV）感染状况及对半胱氨酸天冬氨酸蛋白酶（Caspase） -1 和白细胞介素（IL） -1β表达的影响及临床意义。方法 以 102 例宫颈癌患者（宫颈癌组）、 60 例宫颈上皮内瘤样病变（CIN 组） 及宫颈正常的患者 30 例 （对照组） 为研究对象， PCR-反向点杂交法结合 DNA 芯片技术对各组 hrHPV DNA 进行感染和分型检测。免疫组化 SP 法检测各组 Caspase-1 和 IL-1β的表达。分析宫颈癌患者 hrHPV 感染者 （阳性组）和阴性组、 单一类型感染与多重感染中 2 指标的表达差异。考察 Caspase-1 和 IL-1β表达与宫颈癌患者临床病理参数的关系。结果 宫颈癌组 hrHPV 感染 （阳性组） 75 例 （73.5％）， 检出 hrHPV 11 种， 其中单一类型和多重 hrHPV 感染分别为 61 例（81.3％）和 14 例（18.7％）。宫颈癌组 hrHPV 感染率高于 CIN 组及对照组（36.7％和 6.7％）； Cas⁃ pase-1 和 IL-1β阳性表达率（61.8%和 51.0%）高于对照组（26.7%和 23.3%）， Caspase-1 阳性表达率高于 CIN 组（40.0%， 均 P＜0.01）。宫颈癌患者 hrHPV DNA 表达阴性组的 Caspase-1 和 IL-1β阳性表达率 （77.8%和 74.1%） 均高于相应阳性组 （56.0%和 42.7%）。Caspase-1 和 IL-1β在 hrHPV 单一感染组与多重感染组中阳性表达率差异均无统计学意义 （P＞0.05）。宫颈癌组的 Caspase-1 和 IL-1β蛋白的表达与癌细胞分级、 肿瘤大小、 淋巴结转移及临床分期有关 （P＜0.05 或 P＜0.01）。结论 宫颈癌中存在 hrHPV 单一和多重感染， 且感染率高； hrHPV 可抑制 Caspase-1 和 IL-1β的表达， 促进宫颈癌进展。

关键词: 宫颈肿瘤, 人乳头瘤病毒, 半胱氨酸天冬氨酸蛋白酶 1, 白细胞介素 1β, 炎性体

Abstract: Abstract: Objective To explore the infection status of high risk human papilloma virus (hrHPV) and its effects and clinical significance on expression of cysteinyl aspartate specific protease 1 (Caspase- 1) and interleukin 1 β（IL-1β）in tissues of human cervical carcinoma. Methods A total of 102 patients with cervical carcinoma (cervical carcinoma group), 60 patients with cervical intraepithelial neoplasia (CIN group) and 30 patients with normal cervix (control group) were usedas the research objects. PCR reverse dot hybridization method combined with DNA chip technique were used to detect hrHPV. The expressions of Caspase- 1 and IL-1β were detected by immunohistochemical technique. Data were analyzed between hrHPV positive group and hrHPV negative group, between single type of hrHPV infection group and multiple type of hrHPV infection group. The relationship between caspase-1 and IL-1β expression and clinicopathological parameters in cervical carcinoma patients were observed. Results HrHPV infection was detected in 75 cases（73.5％）in cervical carcinoma group and 11 types of hrHPV were detected. In these 11 cases, single type and multiple type of hrHPV infection were 61 cases（81.3％）and 14 cases（18.7％ ) separately. HrHPV infection rate was much higher in cervical carcinoma group than those in CIN group and control group（36.7％ and 6.7％). Caspase-1 and IL-1β positive rates were significantly higher in cervical carcinoma group（61.8% and 51.0%） than those in control group（26.7% and 23.3%). The positive rate of Caspase-1 was significantly higher in cervical carcinoma group than that in CIN group（40.0%, all P＜0.01). The positive rates of Caspase-1 and IL-1β（77.8% and 74.1%）were higher in hrHPV DNA negative group than those in hrHPV DNA positive group（56.0% and 42.7%). There were no statistical differences in positive rates of Caspase-1 and IL-1β between single type of hrHPV infection group and multiple type of hrHPV infection group（P＞0.05). The difference of positive expressions of Caspase-1 and IL-1β were significantly related with cell differentiation, tumor size, lymphatic metastasis and clinical stage（P＜0.05 or P＜0.01). Conclusion There are single and multiple types of hrHPV infection in cervical carcinoma and the infection rate is high. HrHPVs may promote the progression of cervical carcinoma by restraining the expressions of Caspase-1 and IL-1β.

Key words: cervical carcinoma, human papilloma virus, cysteinyl aspartatespecific protease 1, interleukin 1β, inflammasome