天津医药

天津医药 ›› 2017, Vol. 45 ›› Issue (12): 1341-1344.doi: 10.11958/20170876

• 综述 • 上一篇    

结核分枝杆菌耐异烟肼突变基因及分子 检测方法研究进展

李芊璘 1， 冯福民 1， 戴二黑 2△   

  1. 作者单位： 1 华北理工大学公共卫生学院 （邮编 063210）； 2 华北理工大学附属石家庄市第五医院 作者简介： 李芊璘 （1991）， 女， 硕士在读， 主要从事结核分枝杆菌耐药检测研究 △通讯作者 E-mail：daieh2008@126.com
  • 收稿日期:2017-08-08 修回日期:2017-09-27 出版日期:2017-12-15 发布日期:2017-12-15
  • 通讯作者: 李芊璘 E-mail:moonfesta@126.com
  • 基金资助:
    “十二五”艾滋病和病毒性肝炎等重大传染病防治科技重大专项:基于传染病发生、发展临床分期的血清microRNA表达谱系列诊断试剂盒研发

Progress in isoniazid resistant mutant genes and molecular detection methods of Mycobacterium tuberculosis

LI Qian-lin1, FENG Fu-min1, DAI Er-hei2△   

  1. 1 School of Public Health, North China University of Science and Technology, Tangshan 063210, China; 2 the Fifth Affiliated Hospital of Shijiazhuang, North China University of Science and Technology △Corresponding Author E-mail: daieh2008@126.com
  • Received:2017-08-08 Revised:2017-09-27 Published:2017-12-15 Online:2017-12-15

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摘要： 结核病是一种慢性感染性疾病。我国作为结核病的高负担国家， 其高发率已成为严峻的公共卫生问题。 异烟肼作为治疗结核病的重要一线药物之一， 由于其治疗管理不规范等原因导致临床患者耐药日益严重。目前已 知异烟肼最主要的耐药机制是基因突变， 如何准确有效地检出耐药基因， 是控制结核病的关键。本文从分子角度分 别阐述了异烟肼常见耐药基因 katG、 inhA、 ndh、 ahpC、 oxyR， 以及罕见突变基因 kasA、 sigI、 furA、 mabA、 iniABC、 efpA， 并 针对其检测方法作了概述。

关键词: 分枝杆菌, 结核, 异烟肼, 耐药基因, 分子检测, 综述

Abstract: Tuberculosis is a chronic infection caused by Mycobacterium tuberculosis. The high financial burden and high incidence rate of tuberculosis have become a critical public health problem in China. Isoniazid, as one of the important first-line drugs in the treatment of clinical patients, drug resistance is becoming increasingly serious because of the lack of standard treatment. At present, the main mechanism of isoniazid resistance is known as gene mutation. How to detect drug resistance gene effectively and accurately is the key to control tuberculosis. In this article, we analyzed the frequent genes (katG, inhA, ndh, ahpC and oxyR) and novel genes (kasA, sigI, furA, mabA, iniABC and efpA) from the perspective of molecular biology, and summarized their diagnostic methods.

Key words: Mycobacterium tuberculosis, Isoniazid, drug resistance gene, molecular diagnosis, review