天津医药

天津医药 ›› 2018, Vol. 46 ›› Issue (1): 84-87.doi: 10.11958/20171120

• 临床研究 • 上一篇    下一篇

钙网织蛋白在早期类风湿关节炎中的表达及其与 自身抗体和炎性细胞因子的关系

丁红梅 1△，刘建华 2，贾曙光 1，赵雁艳 1，魏蔚 3，郑芳 4   

  1. 1 河北省唐山市第二医院检验科（邮编 063000），2 手外科；3 天津医科大学总医院风湿免疫科；4 天津医科大学医学检验学院
  • 收稿日期:2017-10-19 修回日期:2017-11-18 出版日期:2018-01-15 发布日期:2018-01-16
  • 通讯作者: 丁红梅 E-mail:dingding3725071@sina.com
  • 作者简介:丁红梅（1980），女，主治医师，博士，主要从事类风湿关节炎发病机制研究
  • 基金资助:
    河北省科技计划项目

Expression of calreticulin and its relationship with autoantibodies and inflammatory cytokines in early rheumatoid arthritis

DING Hong-mei1△，LIU Jian-hua2，JIA Shu-guang1，ZHAO Yan-yan1，WEI Wei3，ZHENG Fang4   

  1. 1 Department of Clinical Laboratory, 2 Department of Hand Surgery, the Second Hospital of Tangshan, Tangshan 063000, China; 3 Department of Rheumatology, Tianjin Medical University General Hospital; 4 School of Laboratory Medicine, Tianjin Medical University
  • Received:2017-10-19 Revised:2017-11-18 Published:2018-01-15 Online:2018-01-16
  • Contact: Hongmei N/ADing E-mail:dingding3725071@sina.com

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摘要： 目的 探讨钙网织蛋白在早期类风湿关节炎（RA）患者体内的表达及其与自身抗体和炎性细胞因子的关 系。方法 收集 106 例早期活动性 RA 患者、95 例经治疗达到稳定期 RA 患者、85 例骨性关节炎（OA）患者和 80 例 健康对照者（HC）的外周血标本。采用酶联免疫吸附试验（ELISA）检测血清钙网织蛋白、抗环瓜氨酸肽抗体（antiCCP）及细胞因子白细胞介素（IL）-1β、IL-6、肿瘤坏死因子（TNF）-α 水平；采用免疫比浊法检测类风湿因子（RF）水 平。Spearman 等级相关分析活动性 RA 患者血清钙网织蛋白水平与血清 IL-1β、IL-6 和 TNF-α 的相关性。结果 早期活动性 RA 组血清钙网织蛋白水平［（5.84±2.62）μg/L］高于稳定期 RA 组［（4.26±1.42）μg/L］、OA 患者组［（3.92± 1.10）μg/L］和 HC 组［（3.86±0.91）μg/L］（P＜0.001），后 3 组的血清钙网织蛋白水平差异无统计学意义（P＞0.05）；早 期活动性 RA 患者 RF 阳性组血清钙网织蛋白水平高于 RF 阴性组［（6.12±2.87）μg/L vs. （4.92±1.22）μg/L，P=0.045］， anti-CCP 阳性组血清钙网织蛋白水平也高于 anti-CCP 阴性组［（6.39±2.93）μg/L vs. （4.69±1.17）μg/L，P=0.002］；早期 活动性 RA 患者血清钙网织蛋白水平与血清 IL-1β（rs=0.386，P=0.009）、IL-6（rs=0.405，P=0.006）、TNF-α（rs=0.428， P=0.003）均呈正相关。结论 钙网织蛋白在早期活动性 RA 患者体内表达升高，且与自身抗体及炎性细胞因子水平 有关，提示其可能成为 RA 早期诊断及预后评估的潜在血清学新指标。

关键词: 关节炎, 类风湿, 白细胞介素 1β, 白细胞介素 6, 肿瘤坏死因子 α, 钙网蛋白, 抗环瓜氨酸肽抗体

Abstract: Objective To investigate the expression of calreticulin and its correlation with autoantibodies and inflammatory cytokines in patients with early rheumatoid arthritis (RA). Methods Serum samples were obtained from 106 patients with early active RA, 95 patients with stable RA, 85 osteoarthritis (OA) and 80 healthy controls (HC). Serum levels of calreticulin, anti - cyclic peptide antibody (CCP), interleukin (IL) - 1β, IL-6 and tumor necrosis factor (TNF) - α were measured by enzyme-linked immnuosorbent assay (ELISA). The serum level of rheumatoid factor (RF) was detected by immunoturbidimetry. The correlations between serum calreticulin and inflammatory cytokines were evaluated using Spearman’s rank correlation test. Results Serum levels of calreticulin were significantly higher in patients with early active RA [(5.84±2.62) μg/L] than those in patients with stable RA [(4.26±1.42) μg/L], patients with OA [(3.92±1.10) μg/L] and HC [(3.86±0.91) μg/L] (P＜0.001). There were no statistical differences in serum levels of calreticulin between stable RA, OA and HC groups (P＞0.05). Serum levels of calreticulin were significantly higher in RF-positive RA patients than those of RF-negative RA patients [(6.12±2.87) μg/L vs. (4.92±1.22) μg/L, P=0.045]. Serum calreticulin levels were also significantly higher in anti-CCP-positive RA patients than those of anti-CCP-negative RA patients [(6.39±2.93) μg/L vs. (4.69±1.17) μg/L, P=0.002]. The serum level of calreticulin was positively correlated with IL-1β (rs=0.386, P=0.009), IL-6 (rs=0.405, P= 0.006) and TNF-α (rs=0.428, P=0.003) in early active RA patients. Conclusion The elevated serum level of calreticulin is related to autoantibodies and inflammatory cytokines in early RA patients, suggesting that calreticulin can be used as a potential biomarker for early diagnosis and prognosis of RA.

Key words: arthritis, rheumatoid, interleukin-1beta, interleukin-6, tumor necrosis factor-alpha, calreticulin, anti-CCP