天津医药

天津医药 ›› 2018, Vol. 46 ›› Issue (1): 38-41.doi: 10.11958/20170921

• 实验研究 • 上一篇    下一篇

CD147 对阿尔茨海默病模型大鼠学习记忆 能力的干预作用

霍会永 1，刘冰 1，曹凌 1，赵现 1，曹妍 1，薛靖 1，王如科 2，李军涛 1△   

  1. 1 邯郸市中心医院神经内二科（邮编 056000），2 神经外科
  • 收稿日期:2017-08-23 修回日期:2017-11-03 出版日期:2018-01-15 发布日期:2018-01-16
  • 通讯作者: 霍会永 E-mail:zhdoctordr@163.com
  • 作者简介:霍会永（1977），男，副主任医师，主要从事神经内科常见疾病研究

Intervention effect of CD147 on learning and memory ability in rat model of Alzheimer’s disease

HUO Hui-yong1, LIU Bing1, CAO Ling1, ZHAO Xian1, CAO Yan1, XUE Jing1, WANG Ru-ke2, LI Jun- tao1△   

  1. 1 Department of Second Neurology, 2 Department of Neurosurgery, Handan Central Hospital, Handan 056000, China
  • Received:2017-08-23 Revised:2017-11-03 Published:2018-01-15 Online:2018-01-16

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摘要： 目的 探讨细胞外基质金属蛋白酶诱导因子（CD147）对阿尔茨海默病模型大鼠学习记忆能力的干预作 用。方法 健康 Sprague-Dawley 大鼠按随机数字表法分为假手术组、模型组和 CD147 组，每组 20 只。所有大鼠 10% 水合氯醛（0.3 g/kg）腹腔注射麻醉，模型组和 CD147 组大鼠于双侧海马 CA1 区注入 10 μg 的 Aβ1～40，假手术组 大鼠于同一部位注射等量的生理盐水。术后 48 h，CD147 组大鼠于双侧脑室注射 CD147 cDNA，模型组和假手术组 大鼠于同一部位注射等量生理盐水。采用 Morris 水迷宫实验对各组大鼠的学习记忆能力进行测试。采用蛋白印迹 法检测 β-淀粉样蛋白（Aβ）和 γ-分泌酶表达。结果 从第 3 天开始，模型组大鼠逃避潜伏期时间较假手术组延长， 而 CD147 组较模型组减少，差异均有统计学意义（P＜0.05）。模型组大鼠经过平台次数和经平台停留时间较假手术 组减少，而 CD147 组较模型组均增加，差异有统计学意义（P＜0.05）。模型组大鼠脑组织 Aβ 和 γ-分泌酶表达较假 手术组增加，而 CD147 组较模型组大鼠减少，差异有统计学意义（P＜0.05）。结论 外源性 CD147 能够显著改善 AD 模型大鼠学习记忆能力，其具体机制可能与调节 γ-分泌酶活性和下调 Aβ 的表达有关。

关键词: 阿尔茨海默病, 记忆, 迷宫学习, 基质金属蛋白酶类, 淀粉样 &beta, 蛋白, γ-分泌酶

Abstract: Objective To investigate the intervention effect of CD147 on learning and memory ability in rat model of Alzheimer’s disease. Methods A total of 60 healthy Sprague-Dawley rats were randomly divided into sham operation group, model group and CD147 group, 20 rats in each group. All of the rats were anesthetized with intraperitoneal injection of 10% chloral hydrate (0.3 g/kg). The rats in the model group and the CD147 group were injected with Aβ1-40 (10 μg) in the bilateral hippocampal CA1 regions, while the rats in the sham operation group were injected with the same amount of saline at the same sites. After 48 h, the rats in CD147 group were injected with CD147 cDNA in the bilateral ventricles, while the rats in model group and sham operation group were injected the same amount of saline at the same sites. Morris water maze test was used to detect the ability of learning and memory of rats. The expressions of β amyloid protein (Aβ) and γ-secretase were detected by Western blot assay. Results The escape latency was significantly longer in model group than that of sham operation group, while which was significantly lower in CD147 group than that of model group (P＜0.05). The number of times across the platform and the time of staying on platform were significantly lower in model group than those of sham operation group, while which was significantly higher in the CD147 group than that of model group (P＜0.05). The expressions of Aβ and γ-secretase were increased significantly in model group compared to those of sham operation group, while which were significantly decreased in CD147 group compared with those of model group (P＜0.05). Conclusion Exogenous CD147 can significantly improve the learning and memory ability of AD rats, and its specific mechanism may be related to regulating the activity of γ-secretase and down regulating the expression of Aβ.

Key words: Alzheimer disease, memory, maze learning, matrix metalloproteinases, amyloid beta-protein, γ-secretase