天津医药

天津医药 ›› 2018, Vol. 46 ›› Issue (3): 259-263.doi: 10.11958/20171251

• 临床研究 • 上一篇    下一篇

叶内型肺隔离征肺部反复感染与基底干细胞增殖功能的关系

王巧兴，吴琦，孙昕，李敏敏 ，宋丽佳，刘会金，陈怀永，李红蔚   

  1. 1天津医科大学总医院（邮编300052）；2天津市海河医院基础医学实验部
  • 收稿日期:2017-11-13 修回日期:2018-01-25 出版日期:2018-03-15 发布日期:2018-03-23
  • 通讯作者: 王巧兴 E-mail:1390941970@qq.com
  • 基金资助:
    国家自然科学基金面上项目（31471121，81773394，81728001）；天津市自然科学基金项目（17JCYBJC24700）；天津市卫计委攻
    关项目（16KG163，16KG164）

The relationship between intralobar pulmonary sequestration syndrome with recurrent infection and basal stem cell proliferation

WANG Qiao-xing, WU Qi, SUN Xin, LI Min-min, SONG Li-jia, LIU Hui-jin, CHEN Huai-yong, Li Hong-wei   

  1. 1 Tianjin Medical University General Hospital, Tianjin 300052, China;2 Department of Basic Medicine, Tianjin Haihe Hospital
  • Received:2017-11-13 Revised:2018-01-25 Published:2018-03-15 Online:2018-03-23

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摘要： 目的 通过研究叶内型肺隔离征（ILS）中基底干细胞的增殖情况探讨 ILS 的发病机制。方法 分别收集4 例 ILS 患者的肺组织和 4 例健康对照肺组织。采用 HE 染色比较 2 组肺组织镜下病理改变，PAS 染色比较 2 组肺组织气道杯状上皮细胞比例，免疫荧光染色及 Real-time PCR 法比较 2 组黏蛋白 MUC5AC 和 MUC5B 的分泌及表达，免疫荧光染色比较纤毛细胞的分布及基底细胞的增殖情况。结果 ILS 组肺组织中充满炎性细胞并有气道上皮的损伤、气道杯状上皮细胞明显增殖、MUC5AC 和 MUC5B 呈高分泌状态。角蛋白 5（KRT5）在 2 组中差异无统计学意义，但增殖抗原 KI67 阳性（KI67+）/KRT5+细胞在 ILS 组明显减少。结论 通过增加气道上皮的增殖来修复并维持气道完整性为未来非外科治疗 ILS 提供了一个新方向。

关键词: 细胞增殖, 黏蛋白类, 感染, 叶内型肺隔离征, 基底干细胞

Abstract: Objective To investigate the proliferation potential of the basal stem cells in intralobar pulmonary sequestration syndrome (ILS) for revealing the pathogenesis of ILS. Methods In this study, lung tissue samples were collected from healthy control subjects (n=4) and abnormal lung lobes of ILS patients (n=4). The pathological changes were compared by HE staining between the two groups. The proportion of goblet cells was compared by PAS staining between the two groups. The expression and secretion of MUC5AC and MUC5B were compared by immunofluorescence staining and realtime PCR between the two groups. The distribution of ciliated cells and the proliferation of basal cells were compared by immunofluorescence staining between the two groups. Results The abnormal lobe of ILS group was filled with inflammatory cells, and the airway epithelium was disrupted. The airway goblet cells of ILS were obviously hyperplastic. The mucin proteins of MUC5AC and MUC5B were hypersecretion in the abnormal lobe of ILS patients. KRT5-positive basal stem cells proliferated only slightly in ILS patients, although there was no significant difference in KRT5 expression between two groups. Conclusion These data suggest that the pathogenesis of ILS may be associated with defects in basal stem cell function. Restoring airway integrity by targeting epithelial regeneration can be a future non-surgical treatment for patients with ILS.

Key words: cell proliferation, mucins, infection, intralobar pulmonary sequestration, basal stem cell