二叶式主动脉瓣患者泛素的表达水平及临床意义

doi:10.11958/20221322

天津医药 ›› 2023, Vol. 51 ›› Issue (9): 935-937.doi: 10.11958/20221322

二叶式主动脉瓣患者泛素的表达水平及临床意义

崔芬芬(), 李元敏^△()

兰州大学第一医院心血管外科（邮编730000）

收稿日期:2022-09-06 修回日期:2023-04-24 出版日期:2023-09-15 发布日期:2023-09-13
通讯作者: △E-mail：ldyylym@163.com
作者简介:崔芬芬（1989），女，主治医师，主要从事重症心脏疾病围手术期监护方面研究。E-mail：cuifenfen1989@126.com
基金资助:
甘肃省青年科技基金计划项目(21JR1RA110)

Expression and clinical significance of ubiquitin in patients with bicuspid aortic valve

CUI Fenfen(), LI Yuanmin^△()

Department of Cardiovascular Surgery, the First Hospital of Lanzhou University, Lanzhou 730000, China

Received:2022-09-06 Revised:2023-04-24 Published:2023-09-15 Online:2023-09-13
Contact: △E-mail：ldyylym@163.com

崔芬芬, 李元敏. 二叶式主动脉瓣患者泛素的表达水平及临床意义[J]. 天津医药, 2023, 51(9): 935-937.

CUI Fenfen, LI Yuanmin. Expression and clinical significance of ubiquitin in patients with bicuspid aortic valve[J]. Tianjin Medical Journal, 2023, 51(9): 935-937.

摘要/Abstract

摘要：

目的分析二叶式主动脉瓣及三叶式主动脉瓣患者瓣膜中泛素的表达水平，探讨泛素与二叶式主动脉瓣之间的相关性。方法收集确诊为二叶式主动脉瓣患者15例，同期选择三叶式主动脉瓣患者15例作为对照组。分别用Western blot及实时荧光定量PCR检测2组患者瓣膜中泛素蛋白及mRNA的表达水平。结果 Western blot结果提示2组患者瓣膜组织均未显示任何阳性信号带，但2组患者瓣膜中均存在泛素mRNA的表达，且二叶式主动脉瓣患者泛素mRNA的表达水平降低（P<0.01）。结论二叶式主动脉瓣中泛素mRNA表达下调，推断泛素可能在二叶式主动脉瓣的发生发展中起一定的作用。

关键词: 泛素, 主动脉疾病, 主动脉瓣关闭不全, 二叶式主动脉瓣, 三叶式主动脉瓣

Abstract:

Objective To analyze the expression of ubiquitin in patients with bicuspid aortic valve (BAV) and tricuspid aortic valve, and to explore the correlation between ubiquitin and BAV. Methods Fifteen patients with BAV were collected, and 15 cases with tricuspid aortic valve were used as controls. The protein and mRNA expression of ubiquitin in valves of the two groups were examined by Western blot assay and real-time fluorescence quantitative PCR. Results Western blot assay indicated that there was no positive signal band in valve tissue in patients of the two groups. However, the mRNA expression of ubiquitin was found in valve of patients with BAV and tricuspid aortic valve. The mRNA expression of ubiquitin was significantly lower in patients with BAV than that of the control group (P<0.01). Conclusion Ubiquitin mRNA expression is significantly down-regulated in BAV, which suggests that ubiquitin might play a role in the development and progression of BAV.

Key words: ubiquitin, aortic diseases, aortic valve insufficiency, bicuspid aortic valve, tricuspid aortic valve

中图分类号:

R654.27

图/表 1

参考文献 19

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二叶式主动脉瓣患者泛素的表达水平及临床意义

Expression and clinical significance of ubiquitin in patients with bicuspid aortic valve

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