丙泊酚麻醉损伤工作记忆编码海马-前额叶皮质网络信息传递的研究

doi:10.11958/20230307

天津医药 ›› 2023, Vol. 51 ›› Issue (9): 938-942.doi: 10.11958/20230307

丙泊酚麻醉损伤工作记忆编码海马-前额叶皮质网络信息传递的研究

郭东勇¹(), 李宝玲², 白文文², 刘迢迢², 徐新宇²^,^△()

1.天津医科大学肿瘤医院麻醉科（邮编300060）
2.天津医科大学生物医学工程与技术学院

收稿日期:2023-03-06 修回日期:2023-05-17 出版日期:2023-09-15 发布日期:2023-09-13
通讯作者: △E-mail：xuxinyu@tmu.edu.cn
作者简介:郭东勇（1977），男，副主任医师，主要从事麻醉对认知的影响方面研究。E-mail：gdy1234@hotmail.com
基金资助:
国家自然科学基金资助项目(61971311)

A study of information transmission in HPC-PFC network during working memory encoding induced by propofol anesthesia

GUO Dongyong¹(), LI Baoling², BAI Wenwen², LIU Tiaotiao², XU Xinyu²^,^△()

1. Department of Anesthesiology, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060, China
2. School of Biomedical Engineering and Technology, Tianjin Medical University

Received:2023-03-06 Revised:2023-05-17 Published:2023-09-15 Online:2023-09-13
Contact: △E-mail：xuxinyu@tmu.edu.cn

郭东勇, 李宝玲, 白文文, 刘迢迢, 徐新宇. 丙泊酚麻醉损伤工作记忆编码海马-前额叶皮质网络信息传递的研究[J]. 天津医药, 2023, 51(9): 938-942.

GUO Dongyong, LI Baoling, BAI Wenwen, LIU Tiaotiao, XU Xinyu. A study of information transmission in HPC-PFC network during working memory encoding induced by propofol anesthesia[J]. Tianjin Medical Journal, 2023, 51(9): 938-942.

摘要/Abstract

摘要：

目的研究丙泊酚麻醉是否损伤大鼠工作记忆编码阶段海马到前额叶皮质神经通路的信息传递。方法 12只大鼠中选取可用于研究分析的6只SD大鼠（3月龄），将16通道微电极阵列分别植入大鼠腹侧海马（vHPC）和内侧前额叶皮质（mPFC）。使用Cerebus信息采集系统记录每只大鼠在接受150 mg/kg丙泊酚麻醉前，麻醉后12、24 h执行工作记忆编码阶段任务时，其mPFC和vHPC这两个责任脑区的多通道局部场电位（LFPs）信号，建立vHPC-mFPC网络，分别计算vHPC和mPFC网络的定向传递函数（DTF）和vHPC-mPFC神经通路信息流，定量表征大鼠麻醉前后vHPC-mPFC神经通路信息传递。结果麻醉后12 h大鼠vHPC和mPFC的平均网络连接强度及vHPC-mPFC信息流均低于麻醉前，而麻醉后24 h vHPC和mPFC的平均网络连接强度及vHPC-mPFC信息流较麻醉前差异均无统计学意义。结论丙泊酚麻醉短暂损伤工作记忆编码vHPC和mPFC网络连接强度和vHPC-mPFC信息传递。

关键词: 二异丙酚, 海马, 大脑皮质, 额叶, 脑电描记术, 工作记忆编码

Abstract:

Objective To figure out whether propofol anesthesia impairs information transmission from hippocampal to prefrontal cortex (HPC-PFC) neural pathways during the coding phase of working memory in rats. Methods Among 12 rats, 6 SD rats (3 months old) were selected for research analysis, and 16-channel microelectrode arrays were implanted into the medial prefrontal cortex (mPFC) and the ventral hippocampus (vHPC) of rats, respectively. The Cerebus information acquisition system was used to record the working memory coding stage tasks of each rat before and 12 and 24 hours after anesthesia with 150 mg/kg propofol. The multichannel local field potential (LFPs) signals of mPFC and vHPC, two responsible brain regions, were used to establish the vHPC-mFPC network. The directional transfer function (DTF) and the information flow of vHPC-mPFC neural pathway in vHPC and mPFC networks were calculated respectively, and the information transmission of vHPC-mPFC neural pathway before and after anesthesia was quantitatively characterized. Results During working memory encoding, information flow from vHPC to mPFC was significantly reduced 12 h after propofol anesthesia comparison with before propofol anesthesia, but it recovered at 24 h after propofol injection. There were no significant differences in the average vHPC and mPFC network connection strength and vHPC-mPFC information flow of rats between before anesthesia and 24 h after anesthesia. Conclusion These results indicated that working memory impairment induced by propofol may result from disrupting information transmission in vHPC-mPFC network during working memory encoding.

Key words: propofol, hippocampus, cerebral cortex, frontal lobe, electroencephalography, working memory encoding

中图分类号:

R318

图/表 6

图1 大鼠行为学实验用Y迷宫

Fig.1 Y maze for rat behavioral experiment

图2 多通道微电极阵列植入mPFC和vHPC示意图

Fig.2 Schematic diagram of multielectrode arrays implanted in mPFC and vHPC

图3 组织学检验结果

Fig.3 Histological result of rat brain

图4 经过预处理获得vHPC和mPFC的局部场电位信号

Fig.4 Average network connection strength of vHPC and mPFC

图5 麻醉前和麻醉后12 h、24 h的DTF比较

Fig.5 Comparison of DTF before anesthesia and 12 h, 24 h after anesthesia. ＊＊P<0.01。

图6 麻醉前和麻醉后12 h、24 h信息流比较

Fig.6 Comparison of information flow before anesthesia and at 12 h, 24 h after anesthesia. ＊＊P<0.01。

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丙泊酚麻醉损伤工作记忆编码海马-前额叶皮质网络信息传递的研究

A study of information transmission in HPC-PFC network during working memory encoding induced by propofol anesthesia

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