天津医药

天津医药 ›› 2026, Vol. 54 ›› Issue (9): 918-922.doi: 10.11958/20253702

• 临床研究 • 上一篇    下一篇

异基因造血干细胞移植后单核细胞亚群及IL-37变化与急性移植物抗宿主病的关系

王洪波(), 王秀娟, 徐建丽, 袁海龙, 江明△()   

  1. 新疆医科大学第一附属医院血液病中心， 新疆维吾尔自治区血液病研究所（邮编 830000）
  • 收稿日期:2025-12-20 修回日期:2026-03-25 出版日期:2026-09-15 发布日期:2026-09-14
  • 通讯作者: △E-mail：jiangmingyyy@126.com
  • 作者简介:王洪波（1990），男，主治医师，主要从事造血干细胞移植、止血与血栓性疾病方面研究。E-mail：528473303@qq.com
  • 基金资助:
    新疆维吾尔自治区自然科学基金青年项目(2022D01C755);新疆维吾尔自治区第三批“天山英才”培养计划青年托举人才项目(2024TSYCQNTJ0049)

The relationship between monocyte subsets and IL-37 changes after allogeneic hematopoietic stem cell transplantation and acute graft-versus-host disease

WANG Hongbo(), WANG Xiujuan, XU Jianli, YUAN Hailong, JIANG Ming△()   

  1. Blood Disease Center of the First Affiliated Hospital of Xinjiang Medical University, Hematology Institute of Xinjiang Uygur Autonomous Region, Urumqi 830000, China
  • Received:2025-12-20 Revised:2026-03-25 Published:2026-09-15 Online:2026-09-14
  • Contact: △E-mail：jiangmingyyy@126.com

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摘要：

目的 探讨异基因造血干细胞移植（allo-HSCT）后外周血单核细胞亚群、白细胞介素-37（IL-37）水平变化及其与急性移植物抗宿主病（aGVHD）发生及严重程度的关系。方法 纳入接受allo-HSCT患者40例，健康体检者（健康对照组）25例，随访至移植后+100 d，根据是否发生aGVHD分为aGVHD组和non-aGVHD组。流式细胞术检测外周血经典（cMo）、中间型（iMo）和非经典（ncMo）单核细胞比例，酶联免疫吸附试验（ELISA）检测血浆IL-37水平。比较3组间上述指标差异，并进行敏感性分析、趋势性检验及相关性分析。结果 3组cMo、iMo比例及IL-37水平差异有统计学意义（均P<0.01），ncMo比例差异无统计学意义（P>0.05）。与健康对照组相比，aGVHD组cMo比例、IL-37降低，iMo比例升高；non-aGVHD组IL-37降低（均Padj<0.05）；aGVHD组iMo比例高于non-aGVHD组（Padj<0.05）。敏感性分析中，aGVHD组较non-aGVHD组iMo比例升高，cMo比例及IL-37下降（均P<0.05）。趋势性检验提示，按健康对照组、non-aGVHD组、aGVHD组顺序，cMo比例、IL-37水平单调下降，iMo比例单调上升（均P<0.05）。相关性分析显示，接受allo-HSCT治疗的恶性血液病患者移植后IL-37与cMo比例呈正相关，与iMo比例呈负相关（均P<0.05）；aGVHD分度与IL-37呈负相关，与iMo比例呈正相关（均P<0.05）。结论 allo-HSCT后外周血出现以iMo比例升高、IL-37下降的免疫改变，与aGVHD发生及严重程度相关。

关键词: 移植物抗宿主病, 异基因造血干细胞移植, 单核细胞, 白细胞介素37

Abstract:

Objective To investigate changes of peripheral blood monocyte subsets and interleukin-37 (IL-37) levels following allogeneic hematopoietic stem cell transplantation (allo-HSCT) and their association with the occurrence and severity of acute graft-versus-host disease (aGVHD). Methods A total of 40 patients underwent allo-HSCT and 25 healthy individuals were enrolled. Patients were followed up to 100 days post-transplantation. Patients were categorized into the aGVHD group and the non-aGVHD group based on whether aGVHD occurred. Peripheral blood samples were collected at the first follow-up visit post-transplantation and at the onset of aGVHD. Flow cytometry was used to detect the proportions of classical (cMo), intermediate (iMo) and non-classical (ncMo) peripheral blood monocytes. Plasma IL-37 levels were measured by enzyme-linked immunosorbent assay (ELISA). The differences of the above indicators were compared between the three groups. The sensitivity analysis, trend tests and correlation analyses were performed. Results There were significant differences in the proportions of cMo and iMo and IL-37 levels among the three groups (all P<0.01), whereas there was no significant difference in the proportion of ncMo (P>0.05). Post-hoc testing showed that lower cMo and IL-37, and higher iMo were found in the aGVHD group compared with those of the control group. IL-37 was lower in non-aGVHD vs controls (all Padj<0.05). Findings were consistent in sensitivity analysis (all P<0.05). Trend analysis indicated that decreasing cMo and IL-37 and increasing iMo were in the three groups (all P<0.05). In patients with hematological malignancies underwent allo-HSCT, IL-37 levels were positively correlated with cMo and negatively correlated with iMo (all P<0.05). In aGVHD group, IL-37 correlated negatively and iMo positively with grade (all P<0.05). Conclusion Following allo-HSCT, peripheral blood exhibits immune alterations characterized by increased iMo proportion and decreased IL-37 levels, which are associated with the occurrence and severity of aGVHD.

Key words: acute graft-versus-host disease, allogeneic hematopoietic stem cell transplantation, monocyte, interleukin-37

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