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小檗胺对视网膜母细胞瘤HXO-RB44细胞增殖的抑制作用

冯亦颖1,王巨存2,潘振华3,李正翔3,李燕2   

  1. 1. 天津市南开医院
    2. 天津市天津医院
    3. 天津医科大学总医院
  • 收稿日期:2011-05-11 修回日期:2011-08-01 出版日期:2012-02-15 发布日期:2012-02-15
  • 通讯作者: 王巨存

Inhibitory effect of berbamine on proliferation of retinoblastoma HXO-RB44 cells

  • Received:2011-05-11 Revised:2011-08-01 Published:2012-02-15 Online:2012-02-15
  • Contact: WANG Ju-Cun

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摘要： 目的： 研究小檗胺对视网膜母细胞瘤细胞株HXO-RB44增殖和细胞周期的影响。方法：对HXO-RB44细胞进行培养和传代，2、4、8、16、32μg/ml小檗胺作用HXO-RB44细胞24h、48 h、72 h后,采用MTT比色法检测细胞增殖活性。4、8、16μg/ml小檗胺作用HXO-RB44细胞24h后，采用流式细胞仪检测细胞周期（PI标记）。结果：与空白对照组比较，小檗胺能显著抑制HXO-RB44细胞的增殖(P < 0.05或P < 0.01)，其抑制作用呈时间-剂量依赖性，其作用24、48、72h的IC50分别为25.26、10.94 和 6.25μg/ml。空白对照组和小檗胺处理组G0/G1期分别为(70.60±1.70)%, (46.43±0.81)%,(46.03±0.29)% 和（42.77±2.44)%；S期分别为(18.90±1.28)%, (20.53±2.50)%, (17.93±0.55 )%和(19.10±2.60)%；G2/M期分别为(10.48±0.97)%, (33.07±1.64)%, (36.03±0.64)%和(38.13±0.35)%。G0/G1期和G2/M期与空白对照组比较,差异具有统计学意义(P < 0.01)。结论：小檗胺在体外可抑制视网膜母细胞瘤HXO-RB44细胞的增殖，并可诱导G2/M期阻滞,可能成为一种有效的抗视网膜母细胞瘤药物。

关键词: 小檗胺, 视网膜母细胞瘤, 细胞增殖, 细胞周期

Abstract: Objective: To study the effect of berbamine (BBM) on the proliferation and cell cycle of retinoblastoma HXO-RB44 cell . Methods: HXO-RB44 cells were cultured and passaged. HXO-RB44 were treated for 24,48 and 72 h with BBM at concentrations of 2,4,8,16 and 32μg/ml，respectively.The proliferation of HXO-RB44 cells was detect by MTT. HXO-RB44 were treated with BBM at concentrations of 4, 8, and 16μg/ml,and collected at 24h . Flow cytometry was used to detect the cell cycle using PI labeled. Results: Berbamine could obviously inhibit the proliferation of HXO-RB44 cells compared with the control group (P < 0.05 or P < 0.01) in a time- and dose-dependent manners.The IC50 value at 24,48 and 72h were 25.26, 10.94 and 6.25μg/ml, respectively. G0/G1 phase of control group and BBM treated group were (70.60±1.70)%, (46.43±0.81)%,(46.03±0.29)% and（42.77±2.44)%, respectively. S phase of control group and BBM treated group were (18.90±1.28)%, (20.53±2.50)%, (17.93±0.55 )% and (19.10±2.60)%, respectively. G2/M phase of control group and BBM treated group were (10.48±0.97)%,(33.07±1.64)%,(36.03±0.64)% and(38.13±0.35)%,respectively.There was a significant difference in G0/G1 and G2/M compared with the control group (P <0.01).Conclusion: Berbamine can inhibit the proliferation of HXO-RB44 cell and induce cell cycle arrest in G2/M phase ,which suggests that berbamine may be an effective anti-retinoblastoma drug.

Key words: Berbamine, Retinoblastoma , Proliferation , Cell cycle