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雌激素对异位、正常子宫内膜细胞EGF mRNA、EGFR mRNA表达的影响

盛威1,尹利荣2,王宇全2,马洪达3   

  1. 1. 天津医科大学第二医院妇产科
    2. 天津医科大学第二医院妇科
    3. 天津医科大学第二医院
  • 收稿日期:2012-06-15 修回日期:2012-10-24 出版日期:2013-04-15 发布日期:2013-04-15
  • 通讯作者: 尹利荣

Efforts of estrogen on the expression of EGF mRNA & EGFR mRNA for the eutopic endometrium cells and endometriosis cells

  • Received:2012-06-15 Revised:2012-10-24 Published:2013-04-15 Online:2013-04-15
  • Contact: YIN Li rong

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摘要：

【摘要】目的 探讨雌激素（E2）对体外培养人正常及异位子宫内膜细胞增殖能力、表皮生长因子（EGF）及其受体（EGFR）mRNA表达的影响。方法 将体外培养的异位内膜细胞和正常内膜细胞分为E2短效组，在培养液中加入 E2 50 nmol/L 30 min；E2长效组，加入E2 50 nmol/L 5 d；对照组，单纯加入培养液。用四甲基偶氮唑盐（MTT）比色法检测各组细胞的增殖能力，同时应用荧光定量PCR技术检测各组细胞中EGF mRNA及EGFR mRNA 的表达水平。结果 经雌激素刺激后，异位及正常内膜细胞的增殖能力增加，细胞中EGF mRNA、EGFR mRNA表达较对照组降低（P < 0.05），但2种细胞的E2短效和长效组间的差异均无统计学意义（P > 0.05）。结论 雌激素可促进分泌期正常和异位内膜细胞的增生及分化，且雌激素作用时间长短并无明显不同。

关键词: 在位内膜细胞, 异位内膜细胞, 雌激素, 表皮生长因子, 表皮生长因子受体, 逆转录聚合酶链反应

Abstract:

[Abstract] Objective  To clarify the effects of estrogen (E2) on the proliferation ability of in vitro cultured human normal and ectopic endometrial cells and the expressions of epidermal growth factor (EGF) and its receptor (EGFR) in these cells. Methods  The in vitro cultured human endometriosis cells and eutopic endometrium cells were both classified into E2 short-term group (with culture fluid stimulated by E2 50 nmol/L for 30 minutes)，E2 long-term group (with culture fluid stimulated by E2 50 nmol/L for 5 days) and control group (with culture fluid only). The MTT method was applied to detect the proliferation ability of endometrial cells, while the expressions of EGF mRNA and EGFR mRNA in these cells was analyzed by PCR techniques. Results  With long-term and short-term stimulation of estrogen, the proliferation ability of eutopic endometrium cells and endometriosis cells and the expression of EGF mRNA and EGFR mRNA were all enhanced compared with those of control (P < 0.05). There were no significant differences in all the three parameters between E2 short-term and E2 long-term groups (P > 0.05). Conclusion  Estrogen can promote the proliferation and differentiation of ectopic endometrial cells and eutopic endometrial cells during their secretary phase. And there was no difference between long-term and short-term stimulation of estrogen.

Key words: Eutopic endometrium cells, Endometriosis cells, Estrogen, EGF, EGFR, RT-PCR