天津医药

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三七皂甙对大鼠肺纤维化的干预作用及机制探讨

郑洪1,蒋萍2,关景芳3,于苏萍3,袁玲3,冯文茹3,陈佳宁1,陶家驹1   

  1. 1. 天津市第一中心医院
    2. 天津市第一中心医院呼吸科
    3. 天津市医药科学研究所
  • 收稿日期:2010-02-24 修回日期:2010-07-26 出版日期:2010-12-15 发布日期:2010-12-15
  • 通讯作者: 郑洪

The intervention and mechanism of PNS on rat pulmonary fibrosis

  • Received:2010-02-24 Revised:2010-07-26 Published:2010-12-15 Online:2010-12-15

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摘要：

目的:观察三七皂甙(PNS)对大鼠肺纤维化的抑制作用并探讨其作用机制。方法:80只大鼠随机分为模型组、PNS组、甲强龙组及PNS与甲强龙联合治疗组。采用博来霉素制备大鼠肺纤维化模型,造模24h后每天给药,分别于7、14、21及28d处死动物,观察其病理变化,检测肺组织转化生长因子(TGF)-β1、细胞黏附分子(ICAM)-1及基质金属蛋白酶(MMP)-9的表达量。结果:PNS组、甲强龙组及联合治疗组均无严重肺泡炎及肺纤维化发生,28d时3个治疗组ICAM-1、TGF-β1及MMP-9的表达水平均较模型组明显降低(P<0.01),联合治疗组TGF-β1表达水平低于2个单药治疗组(P<0.05),PNS组、甲强龙组TGF-β1、ICAM-1表达水平差异无统计学意义(P>0.05)。MMP-9表达水平28d时甲强龙组低于PNS组(P<0.05),而甲强龙组与联合治疗组差异无统计学意义(P>0.05)。结论:PNS能有效抑制博来霉素引起的肺纤维化过程,与糖皮质激素联合应用可减少激素用量及不良反应,同时增强抗纤维化效应。 

关键词: 三七总甙, 肺纤维化, 细胞粘附分子-1, 转化生长因子-β1, 基质金属蛋白酶

Abstract: 【Abstract】 Objective To observe The inhibitory effect of PNS on rat pulmonary fibrosis and to discuss the mechanism of this action.Method Divide 80 only rats randomly into the model group,PNS rental ,methylprednisolone group and the combined treatment group. Adopting bleomycin prepare pulmonary fibrosis in rats, 24 hours later ,give them medication every day,then kill those rats respectively at the 7th、14th、21th、28th day and observe its pathological changes by detecting the expression levels of ICAM-1, TGF-β1, MMP-9 in lung tissue.Results The PNS rental，methylprednisolone group and the combined treatment groups are no serious Alveolitis and pulmonary fibrosis.The expression levels of ICAM-1, TGF-B1, MMP-9 of PNS rental, methylprednisolone group, and the combined treatment group are significantly decrease compared with the model group（p<0.01）. The combined treatment group’s expression levels of TGF-β1 are lower than the two single medicine treatment group(p<0.05).Conclusion the PNS can effectly restrain pulmonary fibrosis process induced by bleomycin,and using corticosteroids together can reduce hormone dosage and side effects, while enhancing the treatment effect.

Key words: PNS, Pulmonary fibrosis, ICAM-1, TGF-B1, MMP-9