天津医药

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羧甲基壳聚糖温敏凝胶对小鼠L929细胞增殖的影响及其生物相容性研究

穆玉   

  1. 河北联合大学冀唐学院
  • 收稿日期:2011-04-14 修回日期:2011-11-12 出版日期:2012-02-15 发布日期:2012-02-15
  • 通讯作者: 穆玉

The effect of carboxymethyl chitosan thermosensitive hydrogel on the L929 cells and the research of its biocompatibility

  • Received:2011-04-14 Revised:2011-11-12 Published:2012-02-15 Online:2012-02-15

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摘要： 目的：自制羧甲基壳聚糖温敏凝胶，研究其对L929细胞增殖的影响并且通过动物实验探讨其组织相容性，为进一步的临床应用提供基础实验依据。方法：用羧甲基壳聚糖与甘油磷酸盐互配, 制备羧甲基壳聚糖温敏凝胶。采用MTT法测定不同浓度的凝胶浸提液对体外培养的L929细胞增殖的影响；将凝胶植入小鼠皮下，然后分别在3、7、14天处死，常规组织切片，HE染色观察凝胶周围组织的炎症反应。结果：培养24h后，各浓度浸提液组对L929细胞的增殖均有一定促进作用，在48h、72h、96h时，各实验组都较阴性及阳性对照组显示出较明显的促进细胞增殖作用，其中0.4倍浸提液组促增殖效果最明显（P＜0.05）；凝胶植入后前3d，实验组以急性炎症为主，随着时间的延长各实验组炎症细胞逐渐减少至消失，说明羧甲基温敏凝胶具有很好的组织相容性。结论：羧甲基壳聚糖温敏凝胶对L929细胞有明显的促进作用且具有良好的组织相容性，在牙周病治疗中具有潜在的应用价值。

关键词: 羧甲基壳聚糖, L929细胞, 牙周病, 温敏凝胶, 增殖

Abstract: Objective: Producing the Carboxymethyl chitosan thermosensitive hydrogel，to research the proliferation of the Carboxymethyl chitosan thermosensitive hydrogel on mouse lung fibroblasts (L929)，moreover, probe into its tissue compatibility through animal experiments which can provide the fundamental experiment proof for further clinical application.Methods: Carboxymethyl chitosan interworked with glycerol phosphate to a preparation of the Carboxymethyl chitosan thermosensitive。Using MTT colorimetric assay to determine the evaluation on proliferation of L929 cells at different concentrations of carboxymethyl chitosan thermosensitive hydrogel.To take the transplant carboxymethyl chitosan temperaturesensitive gel in mice’s subcutaneous tissue, and kill them separately on the third, 7th and 14th day.And make regular tissue slice, then HE dye and observe the inflammatory response on the surrounding tissue of gel, finally evaluate the tissue biocompatibility and degradability of the gel of each group.Results: After 24h，the each concentration of extract groups on L929 cell have a certain role in promoting.In 48h，72h，96h，each experimental group as compared with negative and positive control group showed a more obvious role in promoting cell proliferation，in which 0.4 times leaching liquor have the cell-multiplication function obviously (P <0.05)；On the 3dth after histology injecting, the experimental group is mainly in the acute inflammation.With the time of inflammatory cells in each experimental group gradually decreased to disappearit descriptions that carboxymethyl thermosensitive hydrogels have good biocompatibility.Conclusion:The results suggest that the Carboxymethyl chitosan thermosensitive hydrogel could promote the proliferation of the L929 and have good biocompatibility., local application of the chitosan thermosensitive hydrogel for treating periodontal diseases is feasible.

Key words: Carboxymethyl chitosan, L929 cell, Periodontal disease, Thermosensitive hydrogel, Proliferation