天津医药

• 实验研究 •    

1,25-二羟基维生素D3抑制脂肪细胞分化作用的研究

关晓慧,王君,郭菲,周杰,王宝利   

  1. 天津医科大学代谢病医院内分泌研究所、卫生部激素与发育重点实验室
  • 收稿日期:2013-03-27 修回日期:2013-05-27 出版日期:2013-10-15 发布日期:2013-10-15
  • 通讯作者: 关晓慧

Inhibitory Effects of 1, 25-Dihydroxy-Vitamin D3 on Differentiation of Adipocytes

GUAN Xiao hui,WANG Jun ,GUO Fei ,ZHOU Jie ,WANG Bao li   

  1. Key Laboratory of Hormones and Development (Ministry of Health), Metabolic Diseases Hospital & Institute of Endocrinology, Tianjin Medical University
  • Received:2013-03-27 Revised:2013-05-27 Published:2013-10-15 Online:2013-10-15
  • Contact: GUAN Xiao hui

PDF (PC)

2203

摘要：

【摘要】 目的 研究1,25-二羟基维生素D3[1,25(OH)2D3 ]对脂肪细胞分化的影响及其机制。 方法 以间充质干细胞系C3H10T1/2为研究对象，将其分为6组，分别为对照组、诱导分化组和4个不同剂量的1,25(OH)2D3 处理组。其中对照组加入溶媒，诱导分化组加入脂肪细胞诱导分化试剂， 1,25(OH)2D3 处理组除了加入脂肪细胞诱导分化试剂外，予以10-9、10-8、10-7、10-6mol/L 1,25(OH)2D3处理。处理后5d进行油红O染色，RT-PCR检测脂肪细胞特异性转录因子和Wnt/β-catenin信号通路相关因子表达水平，Western blot检测β-catenin蛋白水平。 结果  1,25(OH)2D3 能够强烈抑制脂肪细胞生成，阻断脂肪细胞特异性转录因子过氧化物酶体增殖物激活受体（PPAR）γ、CCAAT增强子结合蛋白（C/EBP）α及脂肪细胞表征因子aP2mRNA表达，下调Wnt/β-catenin信号通路抑制因子分泌性卷曲相关蛋白1（sFRP1）mRNA，上调β连环素（β-catenin）蛋白水平。结论  1,25(OH)2D3强烈抑制脂肪细胞分化，该作用可能与其激活Wnt/β-catenin信号通路有关。

关键词: 脂细胞, 细胞分化, 骨化三醇, PPARγ, CCAAT增强子结合蛋白α, β连环素, 脂肪细胞表征因子aP2

Abstract:

[Abstract]   Objective   To investigate the effect of1, 25- dihydroxy-vitamin D3(1, 25(OH)2D3 ) on adipocyte differentiation and the underlying mechanism.   Methods   The mesenchymal stem cell line C3H10T1/2was randomly divided into6 groups including control group, differentiation group and4different doses of 1, 25(OH)2D3 groups. The control group wastreated with vehicle. The differentiation group was supplemented with adipocyte differentiation reagent. And the 1, 25(OH)2D3 groups were treated with adipocyte differentiation reagents and10-9, 10-8, 10-7and10-6 mol/L of1, 25(OH)2D3  After culturing for5days, the cells were stained with oil red O, and the expression levels of adipocyte-specific transcription factors and Wnt/ β-catenin signaling pathway related genes were examined by RT-PCR or Western blot methods. Results   1, 25(OH)2D3 significantly reduced the number of differentiated adipocytes and blocked the mRNA levels of adipocyte specific transcription factor PPARγ(peroxisome proliferator-activated receptor gamma), C/EBP α(CCAAT enhancer binding proteinα) and adipocyte characterization factor aP2(fatty acid binding protein 4). These were paralleled by the decreased mRNA expression of Wnt/β-catenin signaling pathway inhibitor sFRP1(Secreted frizzled-related protein 1) and the increased level of β-catenin protein.  Conclusion   1, 25(OH)2D3 inhibits adipocyte differentiation, which may be related to the activation of Wnt/β-catenin signaling.

Key words: adipocytes, cell differentiation, Calcitriol, PPARgamma, CCAAT- enhancer- binding protein- alpha, beta catenin, adipocyte characterization factor aP2