天津医药

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丙泊酚后处理对大鼠脑缺血再灌注损伤的长时程保护作用

李翠1,王国林2,王海云3,罗猛强4   

  1. 1. 天津医科大学总医院麻醉科
    2. 天津医大总医院
    3. 天津医科大学总医院
    4. 天津医科大学
  • 收稿日期:2010-07-09 修回日期:2010-09-20 出版日期:2011-04-15 发布日期:2011-04-15
  • 通讯作者: 李翠

A Long-term Neuroprotective Effect of Propofol Postconditioning on Ischemia-reperfusion Injury in Rats

  • Received:2010-07-09 Revised:2010-09-20 Published:2011-04-15 Online:2011-04-15
  • Contact: li cui

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摘要： 摘要 目的：观察丙泊酚后处理对大鼠脑缺血再灌注损伤是否具有长程保护作用。方法：采用 Longa 法加以改良建立局灶性脑缺血再灌注模型，健康成年 SD 雄性大鼠 144 只，体重 250～280 g，随机分为 4 组：假手术组（S组）、缺血再灌注组（IR组）、内酯对照组（L组）和丙泊酚后处理组（P组），每组36只，各组各取6只在术后1d、3d、7d、14d、21d、28d分别进行改良神经功能缺损程度评分(modified neurological severity score，mNSS)，在术后1d、7d、14d、28d每个时间点每组取6只进行脑梗死体积百分比测定，余每组6只进行Morris水迷宫测试。结果：经长时程的观察，与IR组比较，P组mNSS评分减小，脑梗死体积百分比缩小，水迷宫定位航行测试逃避潜伏期缩短。结论：丙泊酚后处理具有长程脑保护作用，且此作用时效可维持28天，与丙泊酚溶剂内脂成分无关。 关键词 丙泊酚 脑缺血 再灌注损伤 缺血后处理 大鼠

关键词: 丙泊酚, 脑缺血, 再灌注损伤, 缺血后处理, 大鼠

Abstract: Abstract Objective: To observe the long-term effect of propofol on cerebral ischemia-reperfusion injury in rats. Methods: Middle cerebral artery occlusion models were performed in male SD rats. One hundred and forty-four rats were randomly divided into 4 groups with 36 rats in each group, which included S group, IR group, L group and P group. In the following 28 days, the modified neurological severity score, the percentage of cerebral infarct volume and Morris water maze test were detected respectively. Results: After a long-term observation,comparing with IR group, propofol improved the neurological impairment, reduced the cerebral infarct volume and increased the learning and memory ability. Conclusion: Propofol provides a sustained neuroprotective effect, can last for at least 28 days and this effect is not lipid-dependent. Key words propofol cerebral ischemia reperfusion injury postconditioning rats

Key words: propofol, cerebral ischemia, reperfusion injury, postconditioning, rats