天津医药

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钙敏感受体在人骨关节炎软骨中的表达研究

刘军1,曹建刚1,姚庆斌2,李稚君2,付鑫3,马信龙4   

  1. 1. 天津市人民医院
    2. 天津医科大学
    3. 天津市天津医院
    4. 天津医科大学总医院，天津市天津医院
  • 收稿日期:2013-12-24 修回日期:2014-05-12 出版日期:2014-09-15 发布日期:2014-09-15
  • 通讯作者: 马信龙

The expression of the calcium-sensing receptor in human osteoarthritis cartilage

LIU Jun 1,CAO Jiangang 2,YAO Qingbin 3,LI Zhijun 2,FU Xin 4,MA Xinlong 5   

  1. 1. General Hospital of Tianjin Medical University, Tianjin 300052, China;
    2. General Hospital of Tianjin Medical University, Tianjin 300052, China
    3. Basic Medical College of Tianjin Medical University
    4. Department of Joint Surgery, Tianjin Hospital;
    5. General Hospital of Tianjin Medical University, Tianjin 300052, China;Department of Joint Surgery, Tianjin Hospital
  • Received:2013-12-24 Revised:2014-05-12 Published:2014-09-15 Online:2014-09-15
  • Contact: MA Xinlong

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摘要： 【摘要】目的  研究钙敏感受体(CaSR)在人骨性关节炎(OA)软骨中的表达强度，探讨其与OA 发病机制的关 系。方法收集5 例因膝关节OA 接受全膝关节置换术患者的股骨远端及胫骨近端标本，HE 染色后根据Collins 病 理学分级标准进行关节软骨退变分级，Collins 0 级切片8 张为软骨正常组，Collins Ⅱ级切片8 张为软骨退变组，免疫 组织化学染色后比较2 组软骨组织内CaSR 的表达强度。结果 CaSR 在OA 软骨中阳性表达，CaSR 在软骨正常组 的表达强度低于退变软骨组（评分：1.63±0.95 vs 3.52±0.78，t=8.99，P＜0.05）。结论 CaSR 活化与关节软骨退变 有关。 

关键词: 骨性关节炎, 膝关节, 软骨细胞, 钙敏感受体, 免疫组织化学

Abstract:

[Abstract] Objective To investigate calcium-sensing receptor (CaSR) expression level in osteoarthritis (OA) carti?
lage，and the relationship with OA pathogenesis thereof. Methods The degenerated human cartilage of distal femur and
proximal tibia came from 5 patients with OA underwent total knee replacement surgery. The tissue specimens were stained
by HE. According to the Collins histological grading standards, articular cartilage degeneration was classificated in speci?
mens. Eight HE slices of Collins grade 0 were used as normal cartilage group. Eight HE slices of Collins gradeⅡwere used
as degeneration cartilage group. The CaSR expression levels detected by immunohistochemical staining technique were com?
pared between two groups. Results  Expression of CaSR was detected in osteoarthritis cartilage. The expression intensity of
CaSR was significantly lower in normal cartilage group than that of degeneration of cartilage group (score：1.63±0.95 vs 3.52±0.78，t=8.99，P＜0.05). Conclusion  The activation of CaSR is associated with the degeneration of articular cartilage.

Key words: osteoarthritis, knee, articular chondrocytes, calcium-sensing receptor, Immunohistochemistry