天津医药

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色一号对前葡萄膜炎患者外周血Th1/Th2细胞因子的影响

庞雅菊1,郑日中1,梁静2   

  1. 1. 天津市眼科医院
    2. 天津市第四中心医院
  • 收稿日期:2010-04-27 修回日期:2010-07-12 出版日期:2010-10-15 发布日期:2010-10-15
  • 通讯作者: 庞雅菊

Effects of Chinese herbs on Th1/Th2 cytokines in peripheral blood mononuclear cells of the patients with anterior uveitis

  • Received:2010-04-27 Revised:2010-07-12 Published:2010-10-15 Online:2010-10-15

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摘要： 探讨葡萄膜炎患者应用中药治疗前后外周血单核细胞（peripheral blood mononuclear cells，PBMC）培养上清液中Th1/Th2细胞因子水平的变化。方法:应用酶联免疫吸附试验检测41例葡萄膜炎患者中药治疗前后PBMC上清液中干扰素-γ（IFN-γ）、肿瘤坏死因子-α（TNF-α）、白介素-4（IL-4）和白介素-10（IL-10）水平，同期收集30例健康人为对照。结果:治疗前患者PBMC上清液IFN-γ、TNF-α、IL-4和IL-10与治疗后及对照者比较差异有显著性（F=8.96~41.01，P=0.00），其中治疗前IFN-γ和TNF-α明显高于对照者（t=4.93/7.75，P=0.00），IL-4和IL-10与对照者比较差异无显著性（t=1.55/1.46，P>0.05）。治疗后IFN-γ和TNF-α较治疗前降低（t=16.94/21.21，P=0.00），IL-4和IL-10升高（t=6.77/13.04，P=0.00）；治疗后 IFN-γ、IL-4和IL-10均高于对照者（t=2.42~4.32，P=0.02~0.00），TNF-α与对照者比较差异无显著性（t=0.87，P=0.39）。患者治疗前、后IFN-γ与TNF-α呈正相关（r=0.84/0.75，P=0.00），IL-4与IL-10呈正相关（r=0.88/0.86，P=0.00）。结论:葡萄膜炎患者治疗前PBMC中Th1因子明显升高，中药治疗后Th1因子下降，Th2因子升高，表明中药治疗可调节葡萄膜炎患者体内Th1/Th2细胞因子的平衡。

关键词: 葡萄膜炎, 中药, Th1/Th2细胞因子

Abstract: To investigate the effects of on the levels of Thl/Th2 cytokines in the cultured supernatants of the peripheral blood mononuclear cells(PBMC) in the patients with anterior uveitis. Methods:The levels of Th1 cytokines(IFN-γ, TNF-α) and Th2 cytokines(IL-4, IL-10) in the supernatants of PBMC of the 41 cases with anterior uveitis were detected by enzyme linked immunosorbent assay before and after the treatment by using Chinese herbs. 30 cases of healthy persons were collected as controls. Results: The levels of IFN-γ, TNF-α, IL-4 and IL-10 in supernatants of PBMC of the acute patients were different significantly than those of the patients after the treatment and the healthy controls(F=8.96~41.01, P=0.00), the IFN-γ an d TNF-α levels before treatment were higher than thecontrols（t=4.93/7.75，P=0.00），the levels of IL-4 and IL-10 were not different than the controls(t=1.55/1.46, P>0.05). After the treatment with Chinese herbs, the levels of IFN-γ and TNF-α in the recovery patients were decreased than the acute patients(t=16.94/21.21, P=0.00), IL-4 and IL-10 levels were increased(t=6.77/13.04, P=0.00), the levels of IFN-γ, IL-4 and IL-10 were higher than the controls(t=2.42~4.32, P=0.02~0.00), the levels of TNF-α was not different significantly with controls(t=0.87, P=0.39). The IFN-γ and TNF-α levels before and after treatment had positive correlation(r=0.84/0.75, p=0.00), and the IL-4 and IL-10 levels had positive correlation(r=0.88/0.86, P=0.00). Conclusion:The results indicated that the Th1 cytokines were increased in the patients of the acute anterior uveitis. After the treatment with Chinese herbs, the Th1 cytokines were decreased and the Th2 cytokines were increased. Chinese herbs could adjust the balance of Th1/Th2 cytokine of the anterior uveitic patients.

Key words: Uveitis, Chinese herbs, Th1/Th2 cytokines