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利巴韦林抑制K562白血病细胞增殖和诱导其凋亡的研究

袁琳波,杜友爱   

  1. 温州医学院
  • 收稿日期:2010-06-07 修回日期:2010-08-08 出版日期:2010-12-15 发布日期:2010-12-15
  • 通讯作者: 袁琳波

Apoptosis-Inducing effects of ribavirin on K562 leukemia cells

  • Received:2010-06-07 Revised:2010-08-08 Published:2010-12-15 Online:2010-12-15

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摘要： 目的:观察利巴韦林抑制K562白血病细胞增殖和诱导其凋亡的作用。方法:采用四唑盐(MTT)比色实验,集落培养实验观察比较处理组(分别用10-9、10-8、10-7、10-6及10-5mmol/L的利巴韦林处理)K562细胞与对照组(PBS处理)细胞的增殖状态,采用瑞氏-吉姆萨(Wright-Gimesa)染色观察处理组细胞形态学变化,流式细胞术(FCM)检测处理组K562细胞凋亡峰,评价利巴韦林对K562细胞的诱导凋亡作用。结果:利巴韦林抑制K562细胞MTT值和集落产率,随着所加入利巴韦林的浓度的增加,MTT值和集落产率呈递减趋势的剂量依赖关系;利巴韦林可诱导细胞出现亚二倍峰,FCM检测凋亡率为(47.16±2.82)%,并在形态上有诱导凋亡的改变,镜下观察凋亡率为(49.37±0.37)%。结论:利巴韦林有抑制K562细胞增殖和诱导其凋亡的作用。

关键词: 利巴韦林, K562细胞, 增殖, 凋亡

Abstract: Objective To determine the apoptosis-Inducing effects of ribavirin on K562 leukemia cells. Methods Apoptosis-Inducing effects of a neurotransmitter ribavirin were evaluated by proliferation, morphology and detection of apoptosis apex through flow cytometry. We evaluated the effects of ribavirin on K562 cells proliferation by cellular enumeration inMTT assay and clony formation assay, the morphology by Wright-Gimesa(W-G) staining, Results ribavirin inhibited the proliferation of K562 cells, induced the apoptosis apex, and showed apoptosis morphology after drug treatment. Conclusion Ribavirin possessed apoptosis-inducing effects on K562 cells.

Key words: ribavirin, K562 cells, proliferation, apoptosis