天津医药

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吡格列酮对糖尿病大鼠肝脏chemerin、cmklr1及肿瘤坏死因子-α mRNA表达的影响

李国琪   

  1. 天津医科大学代谢病医院
  • 收稿日期:2012-04-27 修回日期:2012-01-13 出版日期:2012-06-15 发布日期:2012-06-15
  • 通讯作者: 李国琪

  • Received:2012-04-27 Revised:2012-01-13 Published:2012-06-15 Online:2012-06-15

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摘要： 摘要 目的：观察噻唑烷二酮类药物对糖尿病大鼠肝脏chemerin、cmklr1及肿瘤坏死因子（TNF）-α mRNA表达的影响。方法：雄性SD大鼠随机分为正常对照组、糖尿病组与吡格列酮组，尾静脉注射链脲佐菌素制作糖尿病大鼠模型，造模成功后吡格列酮组每天按15 mg/kg经胃灌药，连续给药8周。第8周末处死大鼠留取肝脏组织。采用实时定量PCR法检测大鼠肝脏chemerin、cmklr1及TNF-α mRNA的表达水平。结果：糖尿病组肝脏chemerin表达低于正常组及吡格列酮组，差异均有统计学意义（均P <0.017）。3组间肝脏cmklr1表达差异无统计学意义。糖尿病组肝脏TNF-α表达较正常组升高（P <0.017），吡格列酮组较糖尿病组有所降低，但差异无统计学意义。结论：糖尿病大鼠肝脏TNF-α表达增高，吡格列酮可能通过上调chemerin的表达而改善肝脏的胰岛素敏感性。

关键词: 噻唑烷二酮类, 糖尿病, 实验性, 肝, 肿瘤坏死因子α, 大鼠, Sprague-Dawley, 实时定量聚合酶链反应

Abstract: Abstract Objective:To investigate the effect of thiazolidinediones on expression of chemerin,cmklr1 and tumor necrosis factor-alpha mRNA in liver of diabetic rat model.Methods:Murine diabetic models were induced with streptozotocin (STZ). Rats were randomized into normal control group，diabetes group and Pioglitazone group. After successful modeling,the Pioglitazone concentrations of 15 mg/d were given to rats of Pioglitazone group according to 1 kg of rat body weight.Rats were sacrificed after 8 weeks of experiments. At the same time, the liquid nitrogen frozen specimens from liver were taken to use to analyze expressions of chemerin、cmklr1 and tumor necrosis factor-alpha mRNA by the method of real-time quantitative PCR. Results: Results of real-time quantitative PCR showed that expressions of chemerin mRNA was significantly lower in diabetes group than that of normal control group and Pioglitazone group（P <0.017）.No significant difference was found in expressions of cmklr1 mRNA between three groups.The expression of TNF-α mRNA was higer than that of normal control group（P <0.017）. Conclusion:The expression of TNF-α mRNA was upregulated in liver of diabetes group. Pioglitazone may improving insulin sensitivity of liver by upregulating expression of chemerin mRNA

Key words: thiazolidinedionesdiabetes mellitus, experimentallivertumor necrosis factor-alpharats, Sprague-Dawleyreal-time quantitative PCR chemerin cmklr1, 实时定量聚合酶链反应