天津医药

• 实验研究 •    

罗格列酮对重症急性胰腺炎肺损伤保护作用的实验研究

蔡笃雄,曾仕平,汤净,林尤冠   

  1. 海南医学院附属医院消化内科
  • 收稿日期:2012-09-14 修回日期:2013-03-04 出版日期:2013-10-15 发布日期:2013-10-15
  • 通讯作者: 蔡笃雄

An Experimental Study of the Protective Effects of Rosiglitazone on Lung Injury in Severe Acute Pancreatitis

CAI Du xiong,ZENG Shi ping,TANG Jing , LIN You guan   

  1. The Affiliated Hospital of Hainan Medical College
  • Received:2012-09-14 Revised:2013-03-04 Published:2013-10-15 Online:2013-10-15
  • Contact: CAI Du xiong

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摘要：

 【摘要】  目的 探讨罗格列酮对重症急性胰腺炎（SAP）肺损伤的保护作用。  方法 72只SD大鼠随机均分为假手术组（SO组）、SAP组及罗格列酮处理组（干预组）。分别观察各组大鼠血浆淀粉酶（AMY）、肿瘤坏死因子（TNF）-α、动脉血氧分压[p（O2）]、肺组织髓过氧化物酶（MPO）、肺湿/干比值的化及肺组织病理改变，免疫组化法及半定量逆转录聚合酶链反应（RT-PCR）法检测肺组织核因子（NF）-κB、TNF-αmRNA及细胞间黏附分子1（ICAM-1）mRNA的表达。  结果 SAP组AMY、TNF-α、MPO及肺组织NF-κB、TNF-αmRNA及ICAM-1mRNA表达较SO组显著增加(P<0.05），干预组6h、12h肺组织湿/干比值较SAP组降低（P<0.05），而动脉p（O2）较SAP组明显升高（P<0.05），干预组各时点肺组织病理损伤较SAP组均明显减轻。  结论  罗格列酮可能通过抑制肺组织NF-κB的活性，减少肺组织TNF-α、ICAM-1的产生从而保护SAP合并肺损伤。

关键词: 胰腺炎, 急性坏死性, 呼吸窘迫综合征, 成人, NF-κB, 疾病模型, 动物, 大鼠

Abstract:

[Abstract]   Objective   To investigate the protective effects and mechanisms of rosiglitazone on lung injury in severe
acute pancreatitis (SAP).  Methods   Seventy- two SD rats were randomized into three groups: sham operation (SO) group,SAP group and rosiglitazone-pretreated group. The model of SAP was induced by retrograde injection of5% sodium taurocholate into the bili-pancreatic duct in SD rats. Rats of rosiglitazone-pretreated group were given10mg/kg rosiglitazone in?traperitoneally 30min before inducing SAP. The amylase plasma levels, levels of TNF-αandp（O2）, the myeloperoxidase (MPO) and the wet/dry ratio of lung tissue were measured. The expression of NF-κB in pulmonary tissues was assayed by immunohistochemistry. The expressions of TNF-αmRNA and intercellular adhesion molecule1(ICAM-1) mRNA in pulmonary tissues were detected by reverse transcript PCR (RT-PCR). The histopathological changes of pulmonary tissues were evaluated.  Results   Compared with SO group, the plasma levels of amylase and TNF-α, the intrapulmonary MPO were significantly increased in SAP group (P＜0.05). The expression levels of NF-κB, TNF-αmRNA and ICAM-1mRNA in lung tissue were also significantly increased in SAP group (P＜0.01). There were significantly lower levels of the above indicator in rosiglitazone-pretreated group than those of SAP group (P＜0.05). Compared with SO group, the lung wet/dryratio was significantly higher at6h and12h in SAP group (P＜0.05). The values of lung wet/dry ratio were significantly lower at 6h and 12h in rosiglitazone-pretreated group than those of SAP group (P＜0.05). The lung tissue damage was significantly reduced. The level ofp（O2）was significantly decreased in SAP group than that of SO group (P＜0.05). The level of p（O2）was significantly increased at6h and12h in rosiglitazone pretreated group than that of SAP group (P＜0.05).   Conclusion   Rosigli?tazone might have protective effects on SAP-related lung injury by inhibiting NF-κB and decreasing the production of TNF-αand ICAM-1in the lung tissues

Key words: pancreatitis, acute necrotizing, respiratory distress syndrome, adult, NF-kB, disease models, animal, 大鼠