天津医药

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替米沙坦联合氨氯地平对肾性高血压大鼠左室重构的对比研究

马香芹,黄显峰,卢泽恺,张琨,唐滋贵,胡新辉,付国辉,杨红梅   

  1. 河南医学高等专科学校
  • 收稿日期:2013-03-16 修回日期:2013-06-23 出版日期:2013-11-15 发布日期:2013-11-15
  • 通讯作者: 马香芹

A Comparison Study of Combined Medication of Telmisartan with Amlodipine on the Reversal Left Ventricular Remodeling in Renal Hypertensive Rats

MA Xiang qin,HUANG Xian feng,LU Ze kai,ZHANG Kun ,TANG Zi gui,HU Xin hui,FU Guo hui,YANG Hong mei   

  1. Henan Medical College
  • Received:2013-03-16 Revised:2013-06-23 Published:2013-11-15 Online:2013-11-15
  • Contact: MA Xiang qin

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摘要：

【摘要】  目的  比较替米沙坦、氨氯地平以及两者联用治疗肾性高血压大鼠左室重构的效果。 方法  健康雄性SD大鼠50只，随机分成两肾一夹高血压模型组（2KIC组）、假手术组、替米沙坦（10mg/kg）给药组、氨氯地平（2.5mg/kg）给药组、替米沙坦（10mg/kg）+氨氯地平（2.5mg/kg）给药组，每组10只；每周测1次尾动脉压。20周后，采用酶联免疫法检测血浆肾素活性（PRA）、血管紧张素Ⅱ（AngⅡ）、心钠素（ANP）含量。取心脏测定左室质量指数（LVMI），观察心肌电镜超微结构。  结果  2KIC组的收缩压（SBP）、LVMI、PRA，AngⅡ、ANP含量均高于假手术组（P<0.01）；各单用药组的SBP、LVMI、PRA、ANP含量均低于2KIC组（P<0.01），AngⅡ含量高于2KIC组（P<0.01）；联合用药组的SBP、LVMI、AngⅡ、ANP含量明显低于各单用药组（P<0.01），血浆PRA水平差异无统计学意义（P＞0.05）。心肌电镜结果显示联合用药能更显著改善高血压大鼠左室重构。  结论  替米沙坦和氨氯地平均能有效地改善高血压左室重构，两者联用效果更明显。

关键词: 苯甲酸盐类, 苯咪唑类, 氨氯地平, 高血压, 肾性, 心室重构, 替米沙坦

Abstract:

[Abstract]   Objective   To compare the effects of telmisartan and (or) amlodipine on the reversal left ventricular re?
modeling in two-kidney one clip hypertensive rats.  Methods   A total of50healthy male SD rats were randomly divided into5groups (n=10): two-kidney one clip renal hypertensive (2KIC) model group, sham group, telmisartan (10mg/kg) group, am?lodipine (2.5mg/kg) group and telmisartan (10mg/kg) +amlodipine(2.5mg/kg) group. Themodel of two-kidney one clip re?nal hypertensive rats was established. The tail arterial blood pressure was detected once a week. After20weeks, rats were sacrificed and specimens were collected. The left ventricular mass index (LVMI) was assessed. The myocardial ultrastructural changes were observed by electron microscope. Values of plasma renin activity (PRA), angiotensionⅡ (AngⅡ) and atrial natriuretic peptide (ANP) were measured by enzyme linked immunosorbent assay (ELISA).  Results   Compared with sham group, the levels of systolic blood pressure (SBP), LVMI, PRA, AngⅡ and ANP were significantly higher in2KIC group (P<0.01). Compared with2KIC group, values of SBP, LVMI, PRA and ANP were significantly lower in telmisartan group and amlodipine group (P<0.01), but the value of AngⅡwas significantly higher (P<0.01). The levels of SBP, LVMI, AngⅡand ANP were significantly lower in combined medication group than those of single drug medication group (P<0.01). There was no significant difference in the plasma PRA level between those groups (P>0.05). Results of myocardial electron microscopy showed that the left ventricular remodeling was significantly improved in combined treatment group.   Conclusion   Telmisartan and amlodipine can effectively improve the left ventricular remodeling induced by hypertension. There was more effective therapy using both medications together.

Key words: benzoates, benzimidazoles, amlodipine, hypertension, renal, ventricu1ar remodehng, telmisartan