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NF-κBp65和MMP-9在非小细胞肺癌中的表达及意义

杨霞1,徐美林2   

  1. 1. 天津市胸科医院
    2. 天津市胸科医院病理科
  • 收稿日期:2011-05-11 修回日期:2011-06-03 出版日期:2011-09-15 发布日期:2011-09-15
  • 通讯作者: 杨霞

Expression and significance of NF-κBp65 and MMP-9 in non-small cell lung cancer

2   

  • Received:2011-05-11 Revised:2011-06-03 Published:2011-09-15 Online:2011-09-15

摘要: 探讨NF-κBp65和MMP-9蛋白在非小细胞肺癌组织中的表达及其临床病理意义。方法:采用免疫组织化学的方法检测95例非小细胞肺癌(NSCLC组)和27例癌旁正常肺组织(对照组)中NF-κBp65和MMP-9蛋白的表达水平并分析其临床意义。结果:(1)NF-κBp65蛋白在NSCLC癌组织中的阳性表达(44.2%)明显高于癌旁正常肺组织(22.2%),差异有统计学意义(χ2 =4.26,P<0.05)。(2)NF-κBp65在不同组织学分型、分化程度、TNM分期患者表达差异均有统计学意义(P<0.05或P<0.01),而不同年龄、性别及肿瘤大小患者HMGB1蛋白表达率差异均无统计学意义(P>0.05)。 (3) MMP-9蛋白在晚期(Ⅲ期)NSCLC组织中的阳性表达率高于早中期(I-Ⅱ期)者,且差异有统计学意义(P<0.05);在腺鳞癌中的阳性表达率显著高于腺癌和鳞癌(P<0.01),且随着肿瘤分化程度的降低其阳性表达率逐渐升高,差异有统计学意义(P<0.05));另外不同年龄、性别及肿瘤大小患者MMP-9蛋白表达均有差异,但无统计学意义(P>0.05)。(4)NF-κBp65表达情况与MMP-9呈明显正相关(r=0.217,P<0.05),两者的表达均与NSCLC的淋巴结转移有关。结论: NF-κBp65与MMP-9的高表达可能与NSCLC的转移有关。

关键词: 非小细胞肺癌 , NF-κBp65, MMP-9, 转移, 免疫组织化学

Abstract: To investigate the protein expression of nuclear factor kappa B (NF-κB) and MMP-9 in NSCLC tissues and the relationship between their expression and metastasis. Methods: Expression of NF-κBp65 and MMP-9 protein in non-small cell lung cancer tissues (n=95,)and normal tissues(n=27) was detected by immunohistochemical staining. Results:(1)The expression rates of NF-κBp65 protein in NSCLC are 44.2%;In the normal tissues are 22.2%.Significant difference were found for NF-κBp65 expression.(2)The expression of NF-κBp65 protein has significant correlation to tissue typing, clinical classification and clinical stage(P<0.05 or P<0.01), but it has no correlation to age、sex and tumor size (P > 0.05).(3)The expression of MMP-9 protein has significant correlation to tissue typing, clinical classification and clinical stage(P<0.05 or P<0.01), but it has no correlation to age、sex and tumor size (P > 0.05).(4) There was obviously positive correlation between NF-κBp65 and MMP-9( r=0.217,P<0.05).Conclusion:The results indicate that NF-κBp65 and MMP-9 protein play some role in suppression of the metastasis of non-small cell lung cancer.

Key words: NSCLC, NF-κBp65, MMP-9, tumor metastasis, immunohistochemistry