天津医药

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Bcl-2与Caspase-3在溃疡性结肠炎中的表达与意义*

王倩1,吕永慧2,周丽梅1,陈文剑2,江桂英1,江沂1   

  1. 1. 广州市中医医院病理科
    2. 广州市中医医院消化内科
  • 收稿日期:2013-01-29 修回日期:2013-05-02 出版日期:2013-09-15 发布日期:2013-09-15
  • 通讯作者: 王倩

Expressions of Bcl-2and Caspase-3in Ulcerative Colitis

WANG Qian ,LV Yong hui,ZHOU Li mei,CHEN Wen jia, JIANG Gui ying,JIANG Yi   

  1. the Hospital of Chinese Medicine of Guangzhou
  • Received:2013-01-29 Revised:2013-05-02 Published:2013-09-15 Online:2013-09-15
  • Contact: WANG Qian

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摘要：

【摘要】 目的  研究B细胞淋巴瘤-2（Bcl-2）、半胱氨酸天冬氨酸蛋白酶-3（Caspase-3）在溃疡性结肠炎（UC）肠黏膜上皮细胞及固有层炎症细胞内的表达情况及其与UC分期的关系。方法  选择UC活动期、缓解期及正常结肠黏膜组织（对照组）各25例，显微镜观察UC活动期、缓解期的病理形态学特征；采用免疫组织化学SP法检测各组标本上皮细胞和炎症细胞中Bcl-2、Caspase-3的表达情况；并与UC分期进行相关性分析。结果   3组肠黏膜上皮细胞中的Bcl-2、Caspase-3表达差异均无统计学意义；Bcl-2在固有层炎症细胞中表达为活动期组高于对照组（P<0.05），缓解期组与对照组差异无统计学意义；缓解期组的Caspase-3在固有层炎症细胞中的阳性表达级别较高。Bcl-2、Caspase-3与UC分期有关联性（r分别为0.371、0.445）。结论   Bcl-2、Caspase-3与UC的发病密切相关。检测Bcl-2、Caspase-3的表达水平对辅助UC分期具有一定应用价值。

关键词: 半胱氨酸天冬氨酸蛋白酶3, bcl-2相关X蛋白质, 结肠炎, 溃疡性, 上皮细胞, 肠黏膜

Abstract:

[Abstract]  Objective   To study the correlation between the expressions of Bcl-2and Caspase-3in intestinal epithelial and inflammatory cells of lamina propria and the staging and pathogenesis of ulcerative colitis (UC). Methods The  pathomorphological changes of 25 samples of UC in the active phase and remission phase and25samples of control were observed under microscope. The expressions of Bcl-2and Caspase-3were detected by immunohistochemistry SP method. The correlations between expressions of Bcl-2, Caspase-3and UC staging were analyzed. Results   There were no significant differences in expressions of Bcl-2and Caspase-3in intestinal epithelial cells between three groups (P>0.05). The expression of Bcl-2in inflammatory cells of lamina propria was significantly higher in active UC group than that of normal control group(P<0.05), no significant difference between UC remission group and normal control group (P>0.05). There was a higher positive expression of Caspase-3in inflammatory cells of lamina propria in UC remission group. There was correlation between Bcl-2, Caspase-3and UC staging (r=0.371and0.4453respectively). Conclusion   The expression levels of Bcl-2 and Caspase-3were related with pathogenesis of UC. The detection of expression levels of Bcl-2and Caspase-3has a certain application value in UC staging.

Key words: caspase3, bcl-2-associated X protein, Colitis, Ulcerative, epithelial cells, intestinal mucosa