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蓬灰对2型糖尿病大鼠胰腺及骨骼肌IRS-1表达的影响

赵瑞1,谢晓敏2,3   

  1. 1. 宁夏医科大学
    2. 银川市第一人民医院
    3.
  • 收稿日期:2013-04-08 修回日期:2013-05-06 出版日期:2013-09-15 发布日期:2013-09-15
  • 通讯作者: 赵瑞

The Impact of Peng-Hui on the Expression of IRS-1in Pancreas and Skeletal Muscle of Type2Diabetic Rats

ZHAO Rui 1,XIE Xiao min2,3   

  1. 1. Ningxia Medical University
    2. The First People’s Hospital of Yinchuan
    3.
  • Received:2013-04-08 Revised:2013-05-06 Published:2013-09-15 Online:2013-09-15
  • Contact: ZHAO Rui

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摘要：

【摘要】目的   观察不同剂量蓬灰对2型糖尿病（T2DM）大鼠胰腺及骨骼肌中胰岛素受体底物1（IRS-1）表达的影响。方法 将高脂高热卡饲料喂养的大鼠给予腹腔注射小剂量链脲佐菌素（STZ）制作T2DM模型，成模后将大鼠随机分为糖尿病对照组、蓬灰低剂量组、中剂量组和高剂量组。高、中、低剂量组分别给予蓬灰150、100、50mg/kg，另设正常对照组。蓬灰混悬液灌胃8周后，应用免疫组化学方法检测各组大鼠胰腺及骨骼肌IRS-1表达情况，结合血清血糖及胰岛素水平进行分析。结果  中剂量组血糖水平低于糖尿病对照组及低剂量组（P＜0.05）；高、中、低剂量组胰岛素含量与糖尿病对照组比较差异均无统计学意义。高剂量组的胰腺和骨骼肌组织中IRS-1表达水平均低于糖尿病对照组（P＜0.05）。结论  大剂量蓬灰可以降低2型糖尿病大鼠胰腺和骨骼肌组织中IRS-1的表达水平，但短期内可能不会对糖尿病大鼠血糖及血清胰岛素产生影响。

关键词: 糖尿病, 2型, 胰腺, 肌, 骨骼, 血糖, 胰岛素, 大鼠

Abstract:

[Abstract]   Objective  Toobserve the effects of different doses of Peng-Hui on expression levels of insulin receptor
substance1(IRS-1) in type 2diabetes (T2DM) rats.Methods  T2DM rat model was established by feeding with high-fatdiet and intraperitoneal injecting streptozotocin (STZ). T2DM model rats were randomized into four groups: T2DM control group, high dose Peng-Hui (150mg/kg) group, medium dose Peng-Hui (100mg/kg) group and low dose Peng-Hui (50mg/kg) group. The T2DM control group was fed with only high-fat-diet. The normal control group was fed with common diet. The expression levels of IRS-1in pancreas and skeletal muscle tissues were detected by immunohistochemistry method after 8-week treatment, and fasting blood glucose (FBG) and insulin levels were also detected in five groups.Results   FBG levels were significantly lower in medium dose Peng- Hui group than those of T2DM control group and low dose Peng- Hui group (P<0.05). There were no significant differences in insulin levels between high, medium and low dose Peng-Hui groups and T2DM control group (P＞0.05). The expression levels of IRS-1of pancreas and skeletal muscle tissues were significantly lower in high dose Peng-Hui group than that of T2DM control group (P <0.05). Conclusion  The expression of IRS-1 was decreased in high dose Peng-Hui group, but no effects on FBG and plasma insulin.

Key words: diabetes mellitus, type 2, pancreas, muscle, skeletal, blood glucose, insulin, 大鼠